Pramod: [1] Please refrain from posting excessively large (>1MB) attachments to the ccp4bb. Either use a compression technique or use another means of transmitting large files to your recipients without spamming the entire group.
[2] Your predictions are not overlaying well with the spots . Well it may be overlaying well with a subset of spots (in this case, the strongest spots that obey the symmetry *you* chose), but your are leaving out a large number of spots. As I said before, your diffraction pattern is exhibiting strong reflections with weak reflections at a given resolution. It looks as if there are multiple lattices probably due to bad crystal morphology ( a cracked crystal, twinning, pseudosymmetry) or multiple crystals in the beam, the list goes on.... I've experienced your case many, many times.... you'll get a good MR solution, but then the refinement becomes 'stuck'. I think the idiom... 'garbage in, garbage out' applies here.. [3] > > I have given several efforts to the XDS but its giving error "data image of > particular no. does not exist (initially it was saying 11th image than i > change image range then it says 21st and so on) kindly check my data > collection profile and XDS.INP file in attachment' > Sounds like a file name problem. [4] > Or if the crystal is big enough, you could try shooting it in different areas > and 'searching' for a better spot to collect data. > Or 'grow a better crystal'. > > raising the crystals and struggle is on the peak... > There are a number of ccp4bb postings about crystal reproducibility or crystal diffraction improvement. Search the archives for these. F --------------------------------------------- Francis E. Reyes PhD 215 UCB University of Colorado at Boulder
