Careina, If your target is interesting enough, try to reproduce the small crystals in batch and apply for FELS time. Small crystals are actually an advantage there.
Cheers, Jens On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote: > Hi all > > > Any advice on how to get bigger crystals from conditions that give > showers of tiny crystals? I am getting small pretty looking individual > crystals but they are too small and they don't seem to grow. In fact, > in some instances if left for a couple of days they actually dissolve. > I have fiddled around with mother liquor volume, protein concentration > as well as drop volume (I am using hanging drop method) but none seem > to make any difference and I always get the same tiny crystals. I > think I might try microseeding but I haven't tried that yet. > > > Any suggestions or tricks would be welcome > Careina.