'Will' is a fashionable word - but since even a Nobel prize was given based on promises never kept, who would be surprised...
Best wishes from now, Tim On 12/10/2013 10:11 AM, CHAVAS Leonard wrote: > Yes, Tim is right… for now… in few years, with the E-XFEL, we'll get to much > less sample, and much more time available. But that's in few years. > > Leo > > On Dec 10, 2013, at 10:01 AM, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: > >> Dear Careina, >> >> you can also apply for beamtime at PETRA-III. You get away with the same >> size crystals but only require a nano liter drop rather than a few ml of >> your sample. And you probably get beamtime much quicker because all the >> equipment is installed and collecting a data set takes very short time. >> This was demonstrated at the ECM in Warwick this year, so no need for >> FEL (at least for structure determination). >> >> Best, >> Tim >> >> On 12/10/2013 04:36 AM, Jens Kaiser wrote: >>> Careina, >>> If your target is interesting enough, try to reproduce the small >>> crystals in batch and apply for FELS time. Small crystals are actually >>> an advantage there. >>> >>> Cheers, >>> >>> Jens >>> >>> >>> On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote: >>>> Hi all >>>> >>>> >>>> Any advice on how to get bigger crystals from conditions that give >>>> showers of tiny crystals? I am getting small pretty looking individual >>>> crystals but they are too small and they don't seem to grow. In fact, >>>> in some instances if left for a couple of days they actually dissolve. >>>> I have fiddled around with mother liquor volume, protein concentration >>>> as well as drop volume (I am using hanging drop method) but none seem >>>> to make any difference and I always get the same tiny crystals. I >>>> think I might try microseeding but I haven't tried that yet. >>>> >>>> >>>> Any suggestions or tricks would be welcome >>>> Careina. >>> >> >> -- >> Dr Tim Gruene >> Institut fuer anorganische Chemie >> Tammannstr. 4 >> D-37077 Goettingen >> >> GPG Key ID = A46BEE1A >> > -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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