Dear Careina, you can also apply for beamtime at PETRA-III. You get away with the same size crystals but only require a nano liter drop rather than a few ml of your sample. And you probably get beamtime much quicker because all the equipment is installed and collecting a data set takes very short time. This was demonstrated at the ECM in Warwick this year, so no need for FEL (at least for structure determination).
Best, Tim On 12/10/2013 04:36 AM, Jens Kaiser wrote: > Careina, > If your target is interesting enough, try to reproduce the small > crystals in batch and apply for FELS time. Small crystals are actually > an advantage there. > > Cheers, > > Jens > > > On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote: >> Hi all >> >> >> Any advice on how to get bigger crystals from conditions that give >> showers of tiny crystals? I am getting small pretty looking individual >> crystals but they are too small and they don't seem to grow. In fact, >> in some instances if left for a couple of days they actually dissolve. >> I have fiddled around with mother liquor volume, protein concentration >> as well as drop volume (I am using hanging drop method) but none seem >> to make any difference and I always get the same tiny crystals. I >> think I might try microseeding but I haven't tried that yet. >> >> >> Any suggestions or tricks would be welcome >> Careina. > -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
signature.asc
Description: OpenPGP digital signature
