Dear all,
I'm a novice of coot and ccp4. Now I'm doing refinement using both refmac5 and
coot.Here are some problems I'm facing. Really hope you can give me some
suggestions.
1、THE RESOLUTION OF THE DATA IS 2.5 angstrom. After first refinement of refmac5
I got R factor which is 0.26 and R FREE which is 0.31. My question is what the
final R factor and R FREE should be after several rounds of refinement by
refmac5 and coot.
2、At which map level（e/A3 or rmsd）should I refine the data by coot?
3、Can you give me some tips and strategies about how to use coot to decrease R
free? now I just use some basic tricks such as fit density and Ramachandran
plot to refine the data.
Best regards,
Peter Chen
