Dear Jacob,
One of my favorite examples of monovalent cation discrimination is by the enzyme Pyruvate Kinase. It prefers K(+), NH4(+), Rb(+) and Tl+ for maximum catalysis and then activity falls off as the monovalent cation sizes get larger, Cs(+) or smaller Na(+) >> Li (+). The conformations of pyruvate kinase are known to be altered by binding of monovalent cations and it has been studied for over 50 years by a variety of approaches. A number of the X-ray structures are with K(+). I spent a few years of my life studying this enzyme a couple of decades ago. It will hopefully provide some information for your project. Best of luck, Andy Mesecar On Tue, Jan 9, 2018 at 9:42 AM, Keller, Jacob <kell...@janelia.hhmi.org> wrote: > Dear Crystallographers, > > > > Is anyone aware of a soluble protein which changes large-scale > conformation +/- Na+, and is specific for Na+ per se, or at least ignores > K+ and Ca++? E.g., Rb+ or Li+ might be okay. Structural info would be a > plus, but not a sine qua non. > > > > Similarly, what about with K+ or Cl- specificities, but oblivious to > similar common ions? > > > > All the best, > > > > Jacob Keller > > > > +++++++++++++++++++++++++++++++++++++++++++++++++ > > Jacob Pearson Keller > > Research Scientist / Looger Lab > > HHMI Janelia Research Campus > > 19700 Helix Dr, Ashburn, VA 20147 > <https://maps.google.com/?q=19700+Helix+Dr,+Ashburn,+VA+20147&entry=gmail&source=g> > > (571)209-4000 x3159 <(571)%20209-4000> > > +++++++++++++++++++++++++++++++++++++++++++++++++ > > > > The content of this email is confidential and intended for the recipient > specified in message only. It is strictly forbidden to share any part of > this message with any third party, without a written consent of the sender. > If you received this message by mistake, please reply to this message and > follow with its deletion, so that we can ensure such a mistake does not > occur in the future. > > > -- *Andrew D. Mesecar* Head, Department of Biochemistry Walther Professor of Cancer Structural Biology Deputy Director, Purdue Center for Cancer Research E-Mail: amese...@purdue.edu _____________________________________________ *Department of Biochemistry Contact Information:* 175 S. University Street W. Lafayette, IN 47907-2063 765-494-1607 ------------------------------------------------------------------ *Research Lab Contact Information:* Hockmeyer Hall of Structural Biology Room 311 240 S. Martin Jischke Drive West Lafayette, IN 47907-1971 765-494-1924 _________________________________________
