That's very interesting. I guess it's an unusual manifestation of the Hofmeister series.
It might give guidance to developers of screens for both crystallization and cryoEM. Thx, Patrick On 9 January 2018 at 15:44, Andrew Mesecar <amese...@purdue.edu> wrote: > Dear Jacob, > > > > One of my favorite examples of monovalent cation discrimination is by the > enzyme Pyruvate Kinase. It prefers K(+), NH4(+), Rb(+) and Tl+ for maximum > catalysis and then activity falls off as the monovalent cation sizes get > larger, Cs(+) or smaller Na(+) >> Li (+). The conformations of pyruvate > kinase are known to be altered by binding of monovalent cations and it has > been studied for over 50 years by a variety of approaches. A number of the > X-ray structures are with K(+). I spent a few years of my life studying > this enzyme a couple of decades ago. It will hopefully provide some > information for your project. > > > > Best of luck, > > > > Andy Mesecar > > On Tue, Jan 9, 2018 at 9:42 AM, Keller, Jacob <kell...@janelia.hhmi.org> > wrote: > >> Dear Crystallographers, >> >> >> >> Is anyone aware of a soluble protein which changes large-scale >> conformation +/- Na+, and is specific for Na+ per se, or at least ignores >> K+ and Ca++? E.g., Rb+ or Li+ might be okay. Structural info would be a >> plus, but not a sine qua non. >> >> >> >> Similarly, what about with K+ or Cl- specificities, but oblivious to >> similar common ions? >> >> >> >> All the best, >> >> >> >> Jacob Keller >> >> >> >> +++++++++++++++++++++++++++++++++++++++++++++++++ >> >> Jacob Pearson Keller >> >> Research Scientist / Looger Lab >> >> HHMI Janelia Research Campus >> >> 19700 Helix Dr, Ashburn, VA 20147 >> <https://maps.google.com/?q=19700+Helix+Dr,+Ashburn,+VA+20147&entry=gmail&source=g> >> >> (571)209-4000 x3159 <(571)%20209-4000> >> >> +++++++++++++++++++++++++++++++++++++++++++++++++ >> >> >> >> The content of this email is confidential and intended for the recipient >> specified in message only. It is strictly forbidden to share any part of >> this message with any third party, without a written consent of the sender. >> If you received this message by mistake, please reply to this message and >> follow with its deletion, so that we can ensure such a mistake does not >> occur in the future. >> >> >> > > > > -- > *Andrew D. Mesecar* > Head, Department of Biochemistry > Walther Professor of Cancer Structural Biology > Deputy Director, Purdue Center for Cancer Research > E-Mail: amese...@purdue.edu > _____________________________________________ > *Department of Biochemistry Contact Information:* > 175 S. University Street > <https://maps.google.com/?q=175+S.+University+StreetW.+Lafayette,+IN+47907&entry=gmail&source=g> > W. Lafayette, IN 47907 > <https://maps.google.com/?q=175+S.+University+StreetW.+Lafayette,+IN+47907&entry=gmail&source=g> > -2063 > 765-494-1607 > ------------------------------------------------------------------ > *Research Lab Contact Information:* > Hockmeyer Hall of Structural Biology > Room 311 > <https://maps.google.com/?q=311+240+S.+Martin+Jischke+Drive+West+Lafayette,+IN+47907&entry=gmail&source=g> > 240 S. Martin Jischke Drive > <https://maps.google.com/?q=311+240+S.+Martin+Jischke+Drive+West+Lafayette,+IN+47907&entry=gmail&source=g> > West Lafayette, IN 47907 > <https://maps.google.com/?q=311+240+S.+Martin+Jischke+Drive+West+Lafayette,+IN+47907&entry=gmail&source=g> > -1971 > 765-494-1924 > _________________________________________ > > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
