Hello everyone,
I am trying to solve the structure of a protein with a bound ligand at 2.65 A resolution. XDS was used for data reduction, phaser-MR for molecular replacement and Phenix for refinement. The refinement was done with the default settings ( individual B factors, occupancy and TLS parameters). The resultant atomic B factors are quite high. The overall B factor is 82 with a minimum value of 34.57 and maximum of 225.13. I would like to know if any of the data reduction parameters can affect the B factors and how best to deal with this issue. Anandhi ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
