Hi all,
I'm working on a protein that I believe is tetrameric based on AUC, gel
filtration, and crystallography. However, although my asymmetric unit has 4
chains, I cannot form the tetramer within the asymmetric unit. Instead, the
asymmetric unit has two half-tetramers ('dimers'), and each full tetramer is
completed by pairing up with another half-tetramer from a symmetry mate. If I
load this structure into PISA, it recognizes that each of the 'dimers' forms a
stable assembly, but cannot seem to assemble the tetramer. However, if I the
generate symmetry mates in pymol to create a new PDB for the biological
tetramer and give this to PISA, it recognizes a stable tetramer.
Specifically, chains A and C in the original PDB pair with chains A and C of
the second symmetry mate generated in pymol, while chains B and D in the
original pair with chains B and D of the third symmetry mate. How do I use this
knowledge to generate a 3x4 rotation with translation matrix for PDB deposition?
Thanks,
Erik Klontz
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