Try EPPIC (http://eppic-web.org), it will give you all possible symmetric assemblies in your crystal and their constituent interfaces, listing the symmetry operators too. See some help here http://eppic-web.org/ewui/#help
Jose On Sat, 2 Feb 2019 at 16:42, Ricardo Padua <rpa...@brandeis.edu> wrote: > Hi Erik, > > Have you tried Achesym? > > http://achesym.ibch.poznan.pl > > Kowiel, M., Jaskolski, M. & Dauter, Z. (2014). ACHESYM: an algorithm and > server for standardized placement of macromolecular models in the unit cell. > Acta Cryst. D70, doi:10.1107/S1399004714024572. > > Best > > Ricardo Padua > > On Sat, Feb 2, 2019 at 4:06 PM Klontz, Erik <erik.klo...@som.umaryland.edu> > wrote: > >> Hi all, >> >> >> I'm working on a protein that I believe is tetrameric based on AUC, gel >> filtration, and crystallography. However, although my asymmetric unit has 4 >> chains, I cannot form the tetramer within the asymmetric unit. Instead, the >> asymmetric unit has two half-tetramers ('dimers'), and each full tetramer >> is completed by pairing up with another half-tetramer from a symmetry mate. >> If I load this structure into PISA, it recognizes that each of the 'dimers' >> forms a stable assembly, but cannot seem to assemble the tetramer. However, >> if I the generate symmetry mates in pymol to create a new PDB for the >> biological tetramer and give this to PISA, it recognizes a stable tetramer. >> >> >> Specifically, chains A and C in the original PDB pair with chains A and C >> of the second symmetry mate generated in pymol, while chains B and D in the >> original pair with chains B and D of the third symmetry mate. How do I use >> this knowledge to generate a 3x4 rotation with translation matrix for >> PDB deposition? >> >> >> Thanks, >> Erik Klontz >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > -- > Ricardo Padua > HHMI Research Associate > Kern Lab > Dept. of Biochemistry, MS009 > Brandeis University > Waltham, MA 02454, USA > > E-mail: rpa...@brandeis.edu > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
