Hi all,
following up on Kay's point, I think it might be worth to discuss what we as a
community understand by serial crystallography and what makes it
different from multiple crystal crystallography. I recently gave a talk about
multiple-crystal and serial crystallography at a course and I could not
find any textbook definition. Kay's email suggests that my understanding
differs from his...
Best regards,
Selina
On 16/07/2019, 12:04, "CCP4 bulletin board on behalf of Kay Diederichs"
<CCP4BB@JISCMAIL.AC.UK on behalf of kay.diederi...@uni-konstanz.de> wrote:
Hello James,
I like your list, but I wonder how much of this can be covered in
meaningful depth during a single GRC.
Your item
>7) what is the best way to process serial crystallography data?
deserves differentiation; there is a huge gap between serial synchrotron
crystallography (SSX) with rotation of a few degrees, and serial femtosecond
crystallography (SFX) at X-FELs. And stills collected at a synchrotron has
aspects of both techniques.
best,
Kay
On Mon, 15 Jul 2019 19:44:03 +0000, Holton, James M
<jmhol...@slac.stanford.edu> wrote:
>Hello folks,
>
>I have the distinct honor of chairing the next Gordon Research
>Conference on Diffraction Methods in Structural Biology (July 26-31
>2020). This meeting will focus on the biggest challenges currently
>faced by structural biologists, and I mean actual real-world
>challenges. As much as possible, these challenges will take the form of
>friendly competitions with defined parameters, data, a scoring system,
>and "winners", to be established along with other unpublished results
>only at the meeting, as is tradition at GRCs.
>
>But what are the principle challenges in biological structure
>determination today? I of course have my own ideas, but I feel like I'm
>forgetting something. Obvious choices are:
>1) getting crystals to diffract better
>2) building models into low-resolution maps (after failing at #1)
>3) telling if a ligand is really there or not
>4) the phase problem (dealing with weak signal, twinning and
>pseudotranslation)
>5) what does "resolution" really mean?
>6) why are macromolecular R factors so much higher than small-molecule
ones?
>7) what is the best way to process serial crystallography data?
>8) how should one deal with non-isomorphism in multi-crystal methods?
>9) what is the "structure" of something that won't sit still?
>
>What am I missing? Is industry facing different problems than
>academics? Are there specific challenges facing electron-based
>techniques? If so, could the combined strength of all the world's
>methods developers solve them? I'm interested in hearing the voice of
>this community. On or off-list is fine.
>
>-James Holton
>MAD Scientist
>
>
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