Although it sounds as though this condition was not pH adjusted by the company, here are a couple of suggestions that are applicable to ANY commercial screen condition.
1) Try looking at the company's web site for details on how the solution was made, or failing that, try calling the company where you bought the screen and ask (you don't specify the company in your question). 2) Take a small drop of the screen solution from the reservoir well and apply it to pH paper, it'll give you an approximate idea. Don't forget that the protein and the contents of the protein buffer solution will also affect the pH in the mother liquor. Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu (214) 645-6383 (phone) (214) 645-6353 (fax) ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Jon Cooper <00000c2488af9525-dmarc-requ...@jiscmail.ac.uk> Sent: Saturday, October 5, 2019 4:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] A crystallisation screen pH query. WARNING: This email was sent from an external source. Please be cautious of links or attachments, and validate the sender's email address before replying. Does anyone know the pH of JCSG+ condition A3 which is stated as 0.2 M ammonium citrate dibasic, 20% (v/v) PEG3350. I can't really measure it myself, so any help much appreciated! ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
