As was suggested, you can take a small drop and use pH paper to get a rough idea of the pH. The pH will go acidic over time as you have 20% PEG in there. This is an issue with all PEG conditions, so your specific pH will not be known by someone outside your lab as they don't know how old your screen is (and whether you've kept it frozen, in the fridge, at room temp, etc). Best of luck, tom
Tom Peat Proteins Group Biomedical Program, CSIRO 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 tom.p...@csiro.au ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Jon Cooper <00000c2488af9525-dmarc-requ...@jiscmail.ac.uk> Sent: Sunday, October 6, 2019 8:09 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] A crystallisation screen pH query. Does anyone know the pH of JCSG+ condition A3 which is stated as 0.2 M ammonium citrate dibasic, 20% (v/v) PEG3350. I can't really measure it myself, so any help much appreciated! ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
