Hello Jon,
That JCSG+ reagent is a copy of Hampton Research PEG/Ion reagent 48. While there is no pH adjustment made during the formulation of this reagent, the 20% w/v PEG 3350, 0.2 M Ammonium citrate dibasic in deionized water results in a measured pH at 25 degrees Celsius of 5.1. The formulation as well as measured pH, refractive index, conductivity and other details can be found here https://hamptonresearch.com/product-PEG-Ion-PEG-Ion-2-PEG-Ion-HT-11.html under the Support Material(s). Hope this helps.
Kind Regards, Bob Cudney
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Jon Cooper
Sent: Saturday, October 5, 2019 2:10 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] A crystallisation screen pH query.
Does anyone know the pH of JCSG+ condition A3 which is stated as 0.2 M ammonium citrate dibasic, 20% (v/v) PEG3350. I can't really measure it myself, so any help much appreciated!
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