Hi everyone I should mention that DIALS is available (and the developers respond rapidly to any issues raised) and may also be able to do something useful with these images…
Harry > On 2 Dec 2019, at 09:18, Schreuder, Herman /DE <herman.schreu...@sanofi.com > <mailto:herman.schreu...@sanofi.com>> wrote: > > Hi Almuda, > in addition to everything that had been said, I would have a look at all the > images of your data set, to see if there is anything that may be causing > misindexing in addition to the streaky spots. ADXV has the option to play a > movie of a complete data set. Also, ORGX and ORGY must be accurate; as a > crude estimate, the error should be less than half the distance between to > diffraction spots. Also, where Clemens was hinting at, damaged detector > pixels that are not removed from the image may jeopardize the indexing. > Best, > Herman > > Von: Almudena Ponce Salvatierra <maps.fa...@gmail.com > <mailto:maps.fa...@gmail.com>> > Gesendet: Samstag, 30. November 2019 11:00 > An: Schreuder, Herman /DE <herman.schreu...@sanofi.com > <mailto:herman.schreu...@sanofi.com>> > Cc: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK> > Betreff: Re: [EXTERNAL] [ccp4bb] 8/ 2263 spots indexed XDS > > EXTERNAL : Real sender is maps.fa...@gmail.com <mailto:maps.fa...@gmail.com> > > > Hi Herman, > > thank you for your answer. Yes, my data were collected at the synchrotron, > however, the autoprocessing failed with some of the datasets. I have checked > the image headers and the XDS.INP files, trying to find any mismatches > concerning origin, wavelength, etc... but it seems that the information was > recorded properly. I am trying different origins with one of the datasets, > but it has not helped so far. > > I will write back once I find out what the problem was. > > Best wishes, > > Almudena > > El vie., 29 nov. 2019 a las 14:07, Schreuder, Herman /DE > (<herman.schreu...@sanofi.com <mailto:herman.schreu...@sanofi.com>>) escribió: > Dear Almu, > > were your data collected at a synchrotron? Many synchrotrons run automatic > processing of the data being collected and it is worth looking if there is > some processing directory between the frames you collected. If so, these data > may already be quite decent, and in any case, the XDS.INP file may tell you > how to set the optimal processing parameters for this particular beamline. > > You can also look at the refined parameters of your successful XDS run, > especially the ORGX and ORGY. Try to rerun XDS, using the refined parameters > of the successful run. > > Good luck! > Herman > > Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK > <mailto:CCP4BB@JISCMAIL.AC.UK>> Im Auftrag von Almudena Ponce Salvatierra > Gesendet: Freitag, 29. November 2019 12:48 > An: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK> > Betreff: [EXTERNAL] [ccp4bb] 8/ 2263 spots indexed XDS > > EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk > <mailto:owner-ccp...@jiscmail.ac.uk> > > > Dear all, > > I have some data sets that don't want to be processed :p > > In one of them, when I look at IDXREF.LP I see virtually none of the found > spots were indexed and the reason is that they are "too far from the expected > position". The spots are smeary and elongated, so not the prettiest. > > I have managed to process so far only one data set with decent statistics > from another crystal harvested from the same drop, where the diffraction > spots look better. > > I am trying to find in the xds wiki the keyword I should fine tune in order > to make those spots indexable. > > Could you help me please? > > Thank you very much in advance. > > Best wishes, > > Almu > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=OmNNccFfd0ncUhz71B-ZuPSz9zldaXv5adAn5pWyo-0&s=vbh508VYVlXuCpniEp-8Z-n_5rMz1Ae4Tf0jMF2mSWw&e=> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
