Dear Chitra, Try adding a ligand to the crystallization. This worked for us with ALDH7A1:
https://www.ncbi.nlm.nih.gov/pubmed/26260980 ALDH7A1 is an example of an enzyme with a flexible C-terminus (11 residues). The conformation of the C-terminus depends on the presence of a ligand in the substrate site. With the product bound, the C-terminus adopts the “in” conformation (4ZUL). Without the product bound, the C-terminus adopts either the “out” conformation (4ZUK, chains A-F,H) or is disordered (4ZUK, chain G). In this case, the flexibility of the C-terminus is essential for catalytic activity:. https://www.ncbi.nlm.nih.gov/pubmed/29045138 Good luck! Jack John J. Tanner Professor of Biochemistry and Chemistry Associate Chair of Biochemistry University of Missouri 117 Schweitzer Hall 503 S. College Ave. Columbia, MO 65211 Phone: 573-884-1280 Fax: 573-882-5635 Email: tanne...@missouri.edu<mailto:tanne...@missouri.edu> http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html Lab: Schlundt Annex rooms 3,6,9, 203B, 203C Office: Schlundt Annex 203A On Mar 12, 2020, at 8:01 AM, Oganesyan, Vaheh <vaheh.oganes...@astrazeneca.com<mailto:vaheh.oganes...@astrazeneca.com>> wrote: Hi Chitra Latka, By far the best approach is to find what protein is interacting with the one you have the structure and try co-crystallizing them together. At least there will be some more biology (science) involved in what you will be doing. You may get lucky and get different packing of your full length protein and get some sort of structure for last 20 aa. Then what? Regards, From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> On Behalf Of chitra latka Sent: Thursday, March 12, 2020 3:54 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Flexible C terminus Dear All, I am working on a protein that has flexible C terminus. None of the available structures even in homologs have density for C term region (around 20 odd residues). All the available pdb entries have missing density for these 20 residues at C terminus. I am going to try my luck crystallising the entire protein in hope of getting density for C term residues as well (Fingers crossed). Has anyone faced a similar problem where they have managed to get density for a flexible terminus successfully? Any suggestions would be appreciated. Cheers ! Chitra Latka ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://clicktime.symantec.com/3K2tGPDPQHVzmmXDiGLP6PM6H2?u=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
