On 12.03.20 08:53, chitra latka wrote:
Dear All,
I am working on a protein that has flexible C terminus. None of the
available structures even in homologs have density for C term region
(around 20 odd residues). All the available pdb entries have missing
density for these 20 residues at C terminus.
I am going to try my luck crystallising the entire protein in hope of
getting density for C term residues as well (Fingers crossed).
Has anyone faced a similar problem where they have managed to get
density for a flexible terminus successfully?
Any suggestions would be appreciated.
Cheers !
Chitra Latka
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
Dear Chitra
I would nevertheless try. Sometimes flexible termini fold back either in
cis or in trans (crystal packing, a case I just had Yesterday) and you
might learn sth important for biological regulation if you are lucky. At
the same time I would truncate the terminus and crystallize the globular
domain in parallel.
Good luck
Klemens
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
