Dear All,

I am working on a protein that has flexible C terminus. None of the
available structures even in homologs have density for C term region
(around 20 odd residues). All the available pdb entries have missing
density for these 20 residues at C terminus.

I am going to try my luck crystallising the entire protein in hope of
getting density for C term residues as well (Fingers crossed).

Has anyone faced a similar problem where they have managed to get density
for a flexible terminus successfully?

Any suggestions would be appreciated.

Cheers !

Chitra Latka

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