Well, it can still help. I used to be a great fan of inverse-beam expts! Oh, and some of us prefer the word 'multiplicity' ;-0
Jon Cooper
On 23 Jun 2020 22:04, "Peat, Tom (Manufacturing, Parkville)" <tom.p...@csiro.au> wrote:
I would just like to point out that for those of us who have worked too many times with P1 or P21 that even 360 degrees will not give you 'super' anomalous differences.
I'm not a minimalist when it comes to data- redundancy is a good thing to have.
cheers, tom
Tom Peat
Proteins Group
Biomedical Program, CSIRO
343 Royal Parade
Parkville, VIC, 3052
+613 9662 7304
+614 57 539 419
tom.peat@csiro.au
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of 00000c2488af9525-dmarc-request@JISCMAIL.AC.UK <00000c2488af9525-dmarc-request@JISCMAIL.AC.UK>
Sent: Wednesday, June 24, 2020 1:10 AM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] number of frames to get a full dataset?Someone told me there is a cubic space group where you can get away with something like 11 degrees of data. It would be interesting if that's correct. These minimum ranges for data collection rely on the crystal being pre-oriented, which is unheard-of these days, although they can help if someone is nagging you to get off the beam line or if your diffraction fades quickly. Going for 180 degrees always makes sense for a well-behaved crystal, or 360 degrees if you want super anomalous differences. Hope this helps a bit.
Jon Cooper
On 23 Jun 2020 07:29, Andreas Förster <andreas.foerster@DECTRIS.COM> wrote:
Hi Murpholino,
in my opinion (*), the question is neither number of frames nor degrees. The only thing that matters to your crystal is dose. How many photons does your crystal take before it dies? Consequently, the question to ask is How best to use photons. Some people have done exactly that.
All best.
Andreas
(*) Disclaimer: I benefit when you use PILATUS or EIGER - but I want you to use them to your advantage.
On Tue, Jun 23, 2020 at 12:04 AM Murpholino Peligro <murpholinox@gmail.com> wrote:
Hi.
Quick question...
I have seen *somewhere* that to get a 'full dataset we need to collect n frames':at least 180 frames if symmetry is X
at least 90 frames if symmetry is Y
at least 45 frames if symmetry is ZCan somebody point where is *somewhere*?
...also...
what other factors can change n... besides symmetry and radiation damage?
Thanks
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Andreas Förster, Ph.D.Application Scientist Crystallography, Area Sales Manager Asia & Pacific
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