Hello, you could also try: http://www.ic50.tk/kmvmax.html
Or http://ic50.org/kmvmax.html The former has an exponential decay thing that might help with your substrate inhibition and other things if you have biphasic behaviour ;-0 Cheers, Jon.C. Sent from ProtonMail mobile -------- Original Message -------- On 18 Jun 2021, 05:04, Prem Prakash wrote: > Dear all, > Sorry for this off topic. I am working on an enzyme that has an exonuclease > activity. The enzyme preferentially cleaves an unprocessed substrate at a > faster rate than the processed one (known by qualitative analysis). Recently, > I calculated the Vmax, Km and kcat of the enzyme for unprocessed substrate > which are 18.2 pmol/min, 182 nM and 7.1 sec-1 respectively. However, the > Processed substrate has apparently a lower range of Km (not calculated) as > reflected from the curve (because the same increasing concentration range > which is used for unprocessed, shows a steep decline in the initial velocity > of the enzyme with processed substrate. > The latter suggests that Km is way lower than expected. In this case, the > question is, if the Km of processed substrate is way lower than the > Unprocessed, how can we see a faster rate with the former substrate than > later. i.e lower Km and slower rate of cleavage. If it's possible please give > some insights. I have attached the plot comparison between two kinetic assays. > > With kind regards, > > Prem > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
