Hi, I think this paper might be useful for digging into oligomerization and crystallization:
https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201806092 I'm not sure they discuss prokaryotes but do have many interesting ideas. Best, Orly On Fri, Nov 12, 2021 at 7:20 PM Edwin Pozharski <pozharsk...@gmail.com> wrote: > One could use PISA to get a rough distribution of oligomerization states > of proteins in the PDB and compare bacterial vs mammalian... there always > will be a question though of whether any bias is inherent in proteins or > driven by crystallizability itself. > > Personally, I always though that bacterial proteins crystallize better > because they express better and are more stable. All of this is hand > waiving on my part, of course. > > --- > I don't know why the sacrifice didn't work. The science seemed so solid. > Julien XIII, Lord of the Lemurs > > On Fri, Nov 12, 2021 at 9:52 AM Frank von Delft < > frank.vonde...@cmd.ox.ac.uk> wrote: > >> Hello all >> >> Two decades ago, I remember (!) much talk about a reason that bacterial >> proteins crystallize "more easily" is that they tend to come as >> oligomers (dimers and up), and that this internal symmetry made them >> happier to crystallize. >> >> Did anybody ever publish hard evidence? Or even, is there a primary >> citation for the idea? >> >> Thanks >> Frank >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >> available at https://www.jiscmail.ac.uk/policyandsecurity/ >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > -- Orly Avraham, Ph.D. Postdoctoral fellow The lab of Prof. Ora Schueler-Furman The Hebrew University of Jerusalem Israel ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
