Shameless plug, did you try Fusion screen (available from MD). It has been outperforming all the other 22 screens in our bank, either giving exclusive hits or always giving crystals when other screens did too (sure we still had cases when nothing work).
It has shown to be very powerful in our experiences so far in the last 2 years. I would not recommend it otherwise. BW, D > On 8 Nov 2023, at 17:28, MARCHOT Pascale <pascale.marc...@univ-amu.fr> wrote: > > > CAUTION: This email originated from outside of the LMB. > Do not click links or open attachments unless you recognize the sender and > know the content is safe. > .-owner-ccp...@jiscmail.ac.uk-. > ... and reveal by WB if the protein is tagged. > > > > The trickiest part of all this will be to rinse the "xtal(s)" as extensively > as possible in a protein&DNA-free solution before dissolving it for > SDS-PAGE/WB or MS or whichever method you select, in order to avoid > contamination by the protein-DNA complex present in the drop solution. > Paradoxically, the good news is that if the xtal collapses/melts easily > during the rinsing steps, it will mean that it is protein. > > > > Best wishes - Pascale > > > > De : CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> de la part de Jurgen Bosch > <jxb...@case.edu> > Envoyé : mercredi 8 novembre 2023 18:08 > À : CCP4BB@JISCMAIL.AC.UK > Objet : Re: [ccp4bb] What could these crystals be? > > You could also run them on a gel if you don’t want to shot with canons on > small birds. > > Jürgen > > >>> On Nov 8, 2023, at 11:33 AM, M T <michel...@gmail.com> wrote: >>> >>> Dear Careina, >>> >>> If you have easy access to mass spectrometry, you can try to fish/rince >>> your « pumpkin seeds » and send them to mass to try to identify what is >>> inside to see if it needs optimization or not. >>> >>> Best. >>> >>> Le 8 nov. 2023 à 16:03, 000002531c126adf-dmarc-requ...@jiscmail.ac.uk a >>> écrit : >>> >>> >>> Hi all >>> We have been trying with no success to crystalize a protein. Recently we >>> got these strange shape "crystals". They are hard and flat but they do not >>> diffract at all. Any ideas as to what could cause this? >>> Careina >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>> >>> <IMG-20231108-WA0007.jpg> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
