Dear Kavya, Concanavalin A with glucoside involved 120 mg/ml protein. Details of the crystallisation are here:- https://doi.org/10.1016/0022-2836(87)90198-7 PDB code 1GIC. Best wishes, John Emeritus Professor John R Helliwell DSc
> On 5 Feb 2024, at 10:27, kavyashreem <kavyashr...@instem.res.in> wrote: > > > Dear All, > > Has anyone worked on a protein which is highly soluble even at 80mg/ml? > > We have one such candidate, which does not precipitate even at 80mg/ml > instead forms phase separated globules in crystallization plate, which > eventually hardens over a period of 1 to 1.5 months (which is florescent > under UV microscope.) > > We tried screening at different pH, but failed to get any hits. > > Since we got few conditions in which the phase separated globules solidified, > we focused on them and expanded with 120mg/ml protein, still there were not > visible precipitates except for the phase separation. This has been a > challenging target so far. We have tried with different constructs, which > unfortunately are not soluble! > > Does POMs help in such cases? Or do you have any other suggestion. > > Thank you > > Regards > > Kavya > > > > > > CONFIDENTIAL: This email and any files transmitted with it are confidential > and intended solely for the use of the individual or group to whom they are > addressed. If you have received this email in error, please notify the sender > immediately and delete this e-mail from your system. It is NOT AUTHORISED to > copy, forward, or in any way reveal the contents of this message to anyone. > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
