HOD crystallised at 150mg/ml https://www.pnas.org/doi/10.1073/pnas.0909033107
Once I set the wrong time on the centrifuge and it went up to >200 mg/ml. I am sure many others have similar stories. All the best Roberto Roberto A Steiner www.steinerlab.org<http://www.steinerlab.org/> https://twitter.com/steiner_lab roberto.stei...@kcl.ac.uk<mailto:roberto.stei...@kcl.ac.uk> Randall Centre for Cell and Molecular Biophysics Faculty of Life Sciences and Medicine King's College London Room 3.10A New Hunt's House, Guy's Campus SE1 1UL, London, UK Phone 0044 20 78488216 Fax 0044 20 78486435 roberto.stei...@unipd.it<mailto:roberto.stei...@unipd.it> Dipartimento di Scienze Biomediche Università degli Studi di Padova Viale G. Colombo 3 35131 Padova, Italia Telefono 0039 049 8276409 Responses to emails are not expected outside of your normal working hours. On 5 Feb 2024, at 11:27, kavyashreem <kavyashr...@instem.res.in<mailto:kavyashr...@instem.res.in>> wrote: You don't often get email from kavyashr...@instem.res.in<mailto:kavyashr...@instem.res.in>. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> Dear All, Has anyone worked on a protein which is highly soluble even at 80mg/ml? We have one such candidate, which does not precipitate even at 80mg/ml instead forms phase separated globules in crystallization plate, which eventually hardens over a period of 1 to 1.5 months (which is florescent under UV microscope.) We tried screening at different pH, but failed to get any hits. Since we got few conditions in which the phase separated globules solidified, we focused on them and expanded with 120mg/ml protein, still there were not visible precipitates except for the phase separation. This has been a challenging target so far. We have tried with different constructs, which unfortunately are not soluble! Does POMs help in such cases? Or do you have any other suggestion. Thank you Regards Kavya CONFIDENTIAL: This email and any files transmitted with it are confidential and intended solely for the use of the individual or group to whom they are addressed. If you have received this email in error, please notify the sender immediately and delete this e-mail from your system. It is NOT AUTHORISED to copy, forward, or in any way reveal the contents of this message to anyone. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
