For what it is worth, human serum albumin has been crystallized initially from 250 mg/ml solution back in ‘90s. When I started working on ternary complex hSA-FcRn-Fc (PDB Id 4N0U) was afraid that such high concentration couldn’t be achieved with amounts of FcRn I can reasonably express. However, complex got crystallized at around 6-8 mg/ml, if I remember it correctly. What I’m trying to tell is it might be worth trying to find binding partner for your protein and then co-crystallize the complex.
Vaheh Oganesyan, Ph.D. [cid:image001.png@01DA5817.C3AEDD00] R&D | Biologics Engineering One Medimmune Way, Gaithersburg, MD 20878 T: 301-398-5851 vaheh.oganes...@astrazeneca.com<mailto:oganesy...@medimmune.com> From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of kavyashreem Sent: Monday, February 5, 2024 5:27 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Crystallizing a tough target Dear All, Has anyone worked on a protein which is highly soluble even at 80mg/ml? We have one such candidate, which does not precipitate even at 80mg/ml instead forms phase separated globules in crystallization plate, which eventually hardens over a period of 1 to 1.5 months (which is florescent under UV microscope.) We tried screening at different pH, but failed to get any hits. Since we got few conditions in which the phase separated globules solidified, we focused on them and expanded with 120mg/ml protein, still there were not visible precipitates except for the phase separation. This has been a challenging target so far. We have tried with different constructs, which unfortunately are not soluble! Does POMs help in such cases? Or do you have any other suggestion. Thank you Regards Kavya CONFIDENTIAL: This email and any files transmitted with it are confidential and intended solely for the use of the individual or group to whom they are addressed. If you have received this email in error, please notify the sender immediately and delete this e-mail from your system. It is NOT AUTHORISED to copy, forward, or in any way reveal the contents of this message to anyone. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
