Hello This is a common issue with volatile organics in the drop. Solutions that have worked in the past include:
1. covering the drop in oil (some people prefer light oil, I personally prefer heavy/viscous oil, as it makes harvesting easier) 2. diluting the drop with a much larger volume of mother liquor (from the well, or a carefully matched "synthetic" version) 3. gelling the drop with CM-cellulose or some other suitable additive (this also could work with new drops, meaning before your crystals appear - they often grow larger this way) 4. lowering the temperature (the colder, the better) and performing harvest in a cold room 5. flooding the drop with cryo (20-25% ethylene glycol is my #1 choice, but you need multiple drops to play around with different cryos of course) Best of luck! Artem - Cosmic Cats approve of this message On Thu, May 30, 2024 at 7:26 AM 白雪慧 <zb20193020...@cau.edu.cn> wrote: > May I ask scientific researchers, how can they retrieve diffraction data > when encountering a crystal sitting on a droplet and opening a sealed tape > crystal that keeps shaking? Crystals are grown using tert butanol as a > precipitant. As demonstrated in the video. > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
