Dear Paul, Thank you for your response. I wanted to compare a Coulomb potential map to an electron density map. Before aligning these maps, I need to bring them to similar parameters, which requires rescaling one map to match the other. After that, I can proceed with density subtraction.
I hope this clarifies my query. Sorry for any confusion. Thank you again. Regards Devbrat On Wed, Jun 5, 2024, 7:56 PM Paul Emsley <pems...@mrc-lmb.cam.ac.uk> wrote: > > On 05/06/2024 07:00, Devbrat Kumar wrote: > > > -- > > Hello Everyone, > > Hello Devbrat, > > I have a query regarding the resampling of cryoEM density to match crystal > density to obtain a density difference map. Specifically, I am trying to > determine if it is feasible to resample a cryoEM map with an XRD density > map. However, each time I attempt this, the resampling output provides an > arbitrary ASU resample map, resulting in a significant loss of major > density. > > I have been using Coot and Chimera for this process but have not achieved > the desired outcome. Please guide me or suggest how to move forward with > this. My goal is to create an accurate final density difference map. > > > It is not clear to me exactly what the problem is. > > In Coot speak, "resampling" is (merely) changing the grid sampling so that > the map appears (typically) on a finer grid. > > I don't think that this is what you want. > > You want is, I think, "Transform Map by LSQ Model-fit" and if that is what > you used, then I can't help until I am more clear about what you think has > gone wrong. > > Regards, > > Paul. > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/