Dear Tim,
Thank you for your suggestion, it worked well for me!
Best regards,

Christian

Il giorno mar 18 mar 2025 alle ore 11:56 Tim Gruene <[email protected]>
ha scritto:

> On Tue, 18 Mar 2025 09:21:44 +0100
> Christian Buratto <[email protected]> wrote:
>
> > Dear CCP4 community,
> > I am currently trying to determine the structure of a
> > protein-polyoxometalate complex. I am using a polyoxometalate
> > molecule that is a derivative of the one deposited in the CCDC (ICSD:
> > 173439). I am facing difficulties in generating a restraint file for
> > this metal-oxo molecule that can be recognized by Phenix, both for
> > ReadySet and refinement jobs. I have attempted to use ACEdrg and
> > ELBOW, but all my trials have been unsuccessful. A common error in
> > refinement states that Phenix is unable to determine the energy type
> > for Ru or W atoms present in my ligand molecule. If anyone has any
> > suggestions or alternative approaches, I would greatly appreciate
> > your input! Thank you all for your time and help.
> > Best regards,
> > Christian
> >
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> Dear Christian,
>
> sounds like a perfect task for the grade server,
> https://grade.globalphasing.org
> in combination of babel. Usually, I generate a PDB file (e.g. with
> PLATON or SHELXL), then use babel to convert to mol2
> obabel -o mol2 -O yourligand.mol2 yourligand.pdb
>
> and upload the mol2-file to the grade server. This honours your atom
> naming. Make sure the PDB input file includes a residue name (which is
> easily done with SHELXL).
> Cheers,
> Tim
>
> --
> --
> Tim Gruene
> Head of the Core Facility Crystal Structure Analysis
> Faculty of Chemistry
> University of Vienna
>
> Phone: +43-1-4277-70202
>
> https://ccsa.univie.ac.at
>
> GPG Key ID = A46BEE1A
>

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