Dear all,
just for the record, I used IMOD newstack to obtain a file in mode 12,
and gave it a try and implemented reading it in C++ (in gemmi not Coot).
Mode 12 uses half-precision (2-byte) float. Which is supported by C++
since C++23 (as std::float16_t).
If working with older C++, the easiest way is to use a third-party
library for 2-byte float. I used this:
https://sourceforge.net/projects/half
and it seems to work.
Cheers,
Marcin
On Mon, Nov 3, 2025 at 3:52 PM Alexis Rohou <[email protected]> wrote:
>
> Hi Marcin,
>
> CryoSPARC, RELION, cisTEM, ChimeraX, IMOD, Phenix, MOE and (I assume) some
> other packages handle mode 12.
>
> There must be files available that you could download. Or just convert any
> MRC file you already have with IMOD (off the top of my head: newstack -mode
> 12 old.mrc new.mrc).
>
> Cheers,
> Alexis
>
> —
> Alexis Rohou
> Distinguished Scientist
> Director of CryoEM
> Genentech, Department of Structural Biology
> 1 DNA Way
> South San Francisco, CA 94080
> (cell) 781 249 9629
>
>
> Sent from a mobile device, with apologies for brevity, typos, swypos, and
> predictive text errors.
>
>
> On Mon, Nov 3, 2025 at 4:47 AM Marcin Wojdyr <[email protected]> wrote:
>>
>> Hi Alexis,
>> That's a relatively new mode. I don't know any software that handles
>> it. Are there any publically available examples of such files?
>>
>> Marcin
>>
>> On Fri, Oct 31, 2025 at 8:24 PM Alexis Rohou
>> <[email protected]> wrote:
>> >
>> > Hi Paul,
>> >
>> > Unless I'm mistaken, coot doesn't handle MRC maps in mode 12 (16-bit
>> > floats) correctly. At least with coot-1.1.19 I couldn't get such a map to
>> > display, but converting it to mode 2 (32-bit floats) and loading the
>> > converted map works just as expected.
>> >
>> > If that's all correct, I would humbly request that support be added for
>> > mode 12 MRC maps, which I work with very frequently.
>> >
>> > Cheers,
>> > Alexis
>> >
>> > --
>> > Alexis Rohou
>> > Distinguished Scientist
>> > Director of CryoEM
>> > Genentech, Department of Structural Biology
>> > 1 DNA Way
>> > South San Francisco, CA 94080
>> > Cell: 781 249 9629
>> > Pronouns: he/him/his
>> >
>> > ________________________________
>> >
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