The problem is that there is a strong correlation in the two continuous variables due to their large non-zero means. The knee-jerk reaction is to simply demean the variables which is sometimes the right thing to do and sometimes not. In this case, I think it is ok because you are using DOSS. If you were to switch to DODS, it would be the wrong thing to do. Note, you can instruct mri_glmfit to demean the continuous variables by adding the following line to your fsgd file:

DeMeanFlag 1

Again,  you should be careful with this and don't use it with DODS

doug


Nicolas Cherbuin wrote:

Hi,

I am analysing structural data with mri_glmfit. After pre-processing I have 
first used one group variable (gender) with one covariate age. I had no problem 
processing the data and visualising it in tksurfer.

I then tried to add another covariate (measure of immediate recall). So I had 
in total one group variable and two covariates. I reanalysed the data but got 
the following error:

FixVertexAreaFlag = 1
UseMaskWithSmoothing     1
OneSampleGroupMean 0
y    
/media/storage2/proc/freesurfer/60_w2/checked/analysis/lh.gender_age_immrec.thickness.10.mgh
logyflag 0
usedti  0
FSGD gender_age_immrec_fsgd.txt
glmdir lh.gender_age_immrec.glmdir
DoFFx 0
Creating output directory lh.gender_age_immrec.glmdir
Loading y from 
/media/storage2/proc/freesurfer/60_w2/checked/analysis/lh.gender_age_immrec.thickness.10.mgh
Matrix condition is 12866.7
ERROR: matrix is ill-conditioned or badly scaled, condno = 12866.7
Possible problem with experimental design:
Check for duplicate entries and/or lack of range of
continuous variables within a class.
0.000   1.000   68.000   10.000;
1.000   0.000   65.000   5.000;
[trunkated the fsgd file output but it is the same as the one used for the 
sucessful analysis except for one extra column]

After this error I thoroughly checked all my files but could not find anything 
wrong. So to check I could do an analysis using the extra variable I did 
another analysis with gender and immrec. This worked without problem.

I have attached all my fsgd files and mat files. They all use the same subjects 
and gender info and only differ by one column. Since permutations worked I 
cannot fathom why it does not work when all put together.

The mri_glmfit syntax used for each analysis was:

mri_glmfit --y lh.gender_age_immrec.thickness.10.mgh   --fsgd 
gender_age_immrec_fsgd.txt doss  --glmdir lh.gender_age_immrec.glmdir   --surf 
average100 lh --C gender_age_immrec.mat

mri_glmfit --y lh.gender_immrec.thickness.10.mgh   --fsgd 
gender_immrec_fsgd.txt doss  --glmdir lh.gender_immrec.glmdir   --surf 
average100 lh --C gender_immrec.mat

mri_glmfit --y lh.gender_age_immrec.thickness.10.mgh   --fsgd 
gender_age_immrec_fsgd.txt doss  --glmdir lh.gender_age_immrec.glmdir   --surf 
average100 lh --C gender_age_immrec.mat

I use 4.01 (but also checked with 4.02) under Ubuntu 64 bit on a Xeon machine.

Thanks very much for your help

Nic

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------------------------------------------------------------------------

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Class   Female  circle  red     
Variables               age     immrec
Input   60047   Female  68      10
Input   60092   Male    65      5
Input   60094   Female  69      11
Input   60129   Female  69      11
Input   60145   Male    68      11
Input   60155   Female  69      8
Input   60160   Male    66      7
Input   60241   Male    68      4
Input   60286   Female  65      7
Input   60326   Male    65      8
Input   60330   Male    69      11
Input   60345   Female  67      5
Input   60398   Male    65      5
Input   60408   Female  65      7
Input   60440   Male    65      7
Input   60442   Male    68      8
Input   60448   Male    66      6
Input   60452   Female  64      7
Input   60460   Female  69      8
Input   60463   Female  69      11
Input   60472   Female  65      13
Input   60482   Female  65      12
Input   60484   Female  67      15
Input   60491   Female  68      5
Input   60525   Female  65      7
Input   60538   Male    67      9
Input   60540   Female  68      7
Input   60541   Male    64      6
Input   60549   Male    69      13
Input   60553   Female  68      8
Input   60560   Male    67      4
Input   60629   Female  65      7
Input   60631   Female  66      7
Input   60644   Male    65      7
Input   60658   Male    66      12
Input   60664   Male    67      8
Input   60665   Female  64      10
Input   60667   Male    64      7
Input   60682   Female  67      7
Input   60689   Male    67      6
Input   60695   Male    67      6
Input   60697   Male    69      9
Input   60708   Female  67      8
Input   60719   Male    64      7
Input   60726   Male    67      7
Input   60728   Male    65      8
Input   60742   Female  69      5
Input   60751   Female  66      7
Input   60763   Female  69      10
Input   60766   Female  65      8
Input   60776   Female  69      4
Input   60782   Male    66      5
Input   60786   Male    67      7
Input   60788   Male    66      7
Input   60791   Female  67      8
Input   60813   Female  67      8
Input   60834   Male    66      5
Input   60838   Female  65      10
Input   60866   Male    66      12
Input   60876   Male    64      7
Input   60877   Female  68      9
Input   60887   Male    68      9
Input   60891   Female  66      7
Input   60893   Male    68      7
Input   60910   Male    68      6
Input   60913   Female  68      2
Input   60914   Female  67      10
Input   60915   Female  68      7
Input   60917   Female  68      6
Input   60936   Female  66      7
Input   60943   Female  65      5
Input   60966   Female  65      7
Input   60968   Male    67      9
Input   60977   Female  67      5
Input   61021   Female  67      9
Input   61057   Male    69      7
Input   61068   Female  67      7
Input   61083   Female  67      5
Input   61107   Male    64      7
Input   61121   Male    66      4
Input   61124   Male    68      6
Input   61160   Male    67      6
Input   61166   Male    68      7
Input   61187   Male    64      6
Input   61196   Female  66      6
Input   61198   Female  69      9
Input   61200   Female  67      9
Input   61204   Female  69      10
Input   61218   Female  65      7
Input   61225   Female  66      8
Input   61240   Female  66      7
Input   61242   Male    67      6
Input   61247   Male    67      9
Input   61259   Male    68      9
Input   61262   Male    65      4
Input   61270   Male    67      8
Input   61288   Female  69      6
Input   61290   Female  67      10
Input   61303   Male    66      7
Input   61320   Female  66      12
Input   61347   Male    68      6
Input   61358   Female  66      10
Input   61361   Male    66      11
Input   61384   Male    66      8
Input   61392   Female  65      9
Input   61400   Male    65      8
Input   61449   Female  69      9
Input   61478   Male    65      7
Input   61512   Female  67      10
Input   61523   Male    67      6
Input   61525   Male    66      9
Input   61527   Male    66      8
Input   61558   Male    67      9
Input   61564   Female  67      7
Input   61572   Male    69      7
Input   61573   Male    66      5
Input   61577   Female  67      7
Input   61581   Female  66      6
Input   61590   Male    68      6
Input   61611   Female  66      6
Input   61624   Female  66      6
Input   61627   Male    69      6
Input   61635   Male    68      8
Input   61639   Female  65      8
Input   61645   Male    66      7
Input   61650   Male    66      4
Input   61705   Female  65      12
Input   61706   Female  65      8
Input   61730   Male    65      8
Input   61742   Female  65      8
Input   61778   Female  65      8
Input   61782   Male    66      7
Input   61785   Female  65      6
Input   61793   Female  66      7
Input   61795   Female  68      9
Input   61801   Male    66      6
Input   61806   Female  66      6
Input   61830   Female  68      7
Input   61840   Male    66      7
Input   61858   Male    66      5
Input   61878   Male    67      6
Input   61884   Female  65      9
Input   61887   Female  66      5
Input   61895   Male    67      10
Input   61922   Male    64      5
Input   61927   Male    67      11
Input   61937   Female  65      8
Input   61940   Male    67      6
Input   61953   Male    65      9
Input   61958   Female  69      5
Input   61964   Male    69      9
Input   61966   Male    66      7
Input   61978   Male    67      9
Input   61998   Male    65      5
Input   62027   Male    68      6
Input   62044   Male    65      5
Input   62082   Female  66      8
Input   62104   Male    65      7
Input   62113   Female  68      10
Input   62162   Male    66      6
Input   62163   Male    65      7
Input   62181   Female  65      7
Input   62186   Female  65      8
Input   62188   Male    67      8
Input   62194   Male    66      10
Input   62195   Female  65      8
Input   62208   Female  66      10
Input   62242   Female  67      8
Input   62246   Female  65      8
Input   62251   Female  68      6
Input   62256   Male    65      5
Input   62267   Male    65      5
Input   62306   Female  67      6
Input   62346   Male    69      8
Input   62355   Male    68      8
Input   62390   Male    67      4
Input   62395   Female  65      9
Input   62416   Male    67      5
Input   62443   Male    68      8
Input   62465   Male    65      4
Input   62474   Male    64      6
Input   62480   Male    69      6
Input   62484   Male    65      7
Input   62496   Female  64      7
Input   62500   Male    66      6
Input   62516   Male    66      8
Input   62523   Female  66      6
Input   62529   Female  64      7
Input   62538   Male    69      7
Input   62571   Male    65      4
Input   62581   Female  67      8
Input   62585   Male    69      6
Input   62630   Male    67      7
Input   62640   Male    66      8
Input   62649   Female  66      5
Input   62671   Male    65      9
Input   62675   Male    67      10
Input   62685   Male    66      7
Input   62691   Male    69      5
Input   62703   Male    65      6
Input   62713   Female  67      6
Input   62749   Female  66      8
Input   62751   Female  65      6
Input   62768   Male    67      8
Input   62770   Female  69      8
Input   62773   Female  67      5
Input   62793   Female  68      7
Input   62841   Male    65      7
Input   62848   Male    66      5
Input   62850   Male    67      4
Input   62858   Female  68      5
Input   62866   Male    67      8
Input   62916   Female  66      8
Input   62920   Female  67      8
Input   62935   Female  68      6
Input   62955   Male    68      5
Input   62965   Female  65      9
Input   62975   Male    65      9
Input   63001   Male    66      7
Input   63010   Female  65      10
Input   63015   Male    68      7
Input   63026   Female  68      6
Input   63028   Female  65      10
Input   63035   Female  66      9
Input   63037   Male    67      6
Input   63040   Female  65      8
Input   63044   Female  65      9
Input   63053   Male    67      5
Input   63056   Male    66      6
Input   63068   Male    66      8
Input   63081   Female  67      8
Input   63121   Male    65      7
Input   63136   Male    65      7
Input   63187   Female  67      7
Input   63194   Male    65      6
Input   63218   Male    67      6
Input   63329   Female  67      4
Input   63348   Female  66      8
Input   63365   Male    68      7
Input   63407   Female  66      8
Input   63411   Male    67      7
Input   63429   Male    66      9
Input   63433   Female  65      5
Input   63434   Male    65      8
Input   63435   Female  68      8
Input   63450   Female  68      6
Input   63470   Male    68      6
Input   63471   Male    66      8
Input   63473   Male    67      8
Input   63490   Female  68      9
Input   63491   Female  69      6
Input   63492   Male    68      6
Input   63534   Female  64      12
Input   63545   Female  65      6
Input   63568   Female  69      10
Input   63575   Male    66      5
Input   63586   Female  67      5
Input   63636   Male    65      7
Input   63637   Male    66      5
Input   63649   Male    66      10
Input   63674   Female  69      8
Input   63693   Female  65      12
Input   63750   Female  65      7
Input   63755   Female  68      8
Input   63766   Male    66      6
Input   63770   Male    67      6
Input   63773   Male    65      4
Input   63801   Female  65      8
Input   63819   Male    64      2
Input   63842   Female  67      6
Input   63860   Female  67      7
Input   63870   Male    67      5
Input   63897   Female  65      6
Input   63909   Female  67      6
Input   63943   Female  68      9
Input   63973   Female  68      8
Input   63977   Female  67      7
Input   63988   Female  66      6
Input   64058   Male    65      6
Input   64060   Female  69      5
Input   64079   Female  68      9
Input   64092   Female  68      8
Input   64098   Male    65      7
Input   64110   Male    69      6
Input   64119   Female  67      4
Input   64123   Female  66      10
Input   64129   Male    68      7
Input   64186   Male    70      8
Input   64206   Female  68      9
Input   64212   Male    66      6
Input   64227   Female  68      10
Input   64243   Male    69      9
Input   64250   Male    66      4
Input   64264   Male    69      7
Input   64297   Female  67      7
Input   64310   Male    69      10
Input   64312   Female  66      9
Input   64318   Male    65      6
Input   64336   Female  67      7
Input   64346   Male    65      5
Input   64350   Male    66      6
Input   64370   Female  67      4
Input   64376   Male    68      7
Input   64387   Male    65      8
Input   64394   Female  69      7
Input   64406   Male    65      7
Input   64415   Male    65      6
Input   64431   Male    69      9
Input   64432   Female  64      7
Input   64469   Female  68      8
Input   64475   Male    64      7
Input   64488   Female  66      7
Input   64504   Male    68      7
Input   64531   Female  64      6
Input   64537   Female  64      6
Input   64542   Female  65      8
Input   64552   Female  67      11
Input   64590   Male    66      3
Input   64592   Female  68      7
Input   64596   Male    66      9
Input   64620   Male    69      9
Input   64625   Male    67      7
Input   64640   Male    64      8
Input   64657   Female  66      6
Input   64668   Female  66      4
Input   64680   Male    66      7
Input   64691   Male    67      7
Input   64706   Male    69      4
Input   64734   Male    67      4
Input   64745   Female  66      7
Input   64764   Female  66      7
Input   64781   Male    66      10
Input   64797   Male    66      7
Input   64798   Male    68      7
Input   64806   Male    67      8
Input   64812   Male    66      7
Input   64818   Male    66      9
Input   64824   Female  65      6
Input   64829   Male    67      8
Input   64843   Female  67      9
Input   64867   Female  68      6
Input   64868   Male    68      7
Input   64876   Male    68      5
Input   64884   Female  68      7
Input   64886   Male    69      6
Input   64897   Female  68      10
Input   64899   Male    65      4
Input   64910   Male    65      6
Input   64913   Female  68      12
Input   64937   Male    68      5
Input   64947   Female  65      9
Input   64961   Female  68      8
Input   64987   Male    67      11
Input   64997   Male    68      5
DefaultVariable         age                     
------------------------------------------------------------------------


$Id: mri_glmfit.c,v 1.138.2.1 2007/09/12 15:38:19 nicks Exp $
cwd /media/storage2/proc/freesurfer/60_w2/checked/analysis
cmdline mri_glmfit --y lh.gender_age_immrec.thickness.10.mgh --fsgd gender_age_immrec_fsgd.txt doss --glmdir lh.gender_age_immrec.glmdir --surf average100 lh --C gender_age_immrec.mat sysname Linux
hostname mri-lab
machine  x86_64
user     u3184049
FixVertexAreaFlag = 1
UseMaskWithSmoothing     1
OneSampleGroupMean 0
y    
/media/storage2/proc/freesurfer/60_w2/checked/analysis/lh.gender_age_immrec.thickness.10.mgh
logyflag 0
usedti  0
FSGD gender_age_immrec_fsgd.txt
glmdir lh.gender_age_immrec.glmdir
DoFFx 0
------------------------------------------------------------------------

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Douglas N. Greve, Ph.D.
MGH-NMR Center
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