Hi Ping - It looks like the path initialization may have failed. The 
algorithm needs an initial guess for the path and iterates from there. 
This initial guess is chosen based on the tracts in the atlas and if your 
subject is not perfectly aligned with the atlas this initial guess might 
end up going off the white matter for example. Does the blue line in your 
snapshot look like it may not coincide well with you subject's CST, going 
off the white matter close to the end, etc?

a.y

On Thu, 22 Sep 2011, Ping-Hong Yeh wrote:

> Hello,
>
> The segmented left CST tracts of one of our 3T data was not
> satisfactory, which has only few voxels with 1000 gray values (see
> attached snapshot pictures for "trac-all" and "cst_prob"). The
> manually tracking using deterministic tractography seems OK (see
> cst_stremline). Any suggestions on which step may go wrong in
> trac-all?
>
> Thank you,
>
> Ping
>
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