That is the correct matrix to use. You can create a new FSGD file (and 
the second matrix would be correct for it), but you do not have to. It 
will save you a few degrees of freedom.
doug



On 09/07/2012 05:49 AM, Meng Li wrote:
> Hi professor,
> Thanks for your reply.
> When I perform the main effect of dignosis, is the following matrix I 
> used correct?
> 0.25 0.25 0.25 0.25 -0.25 -0.25 -0.25 -0.25 0 0 0 0 0 0 0 0 0 0 0 0 0 
> 0 0 0 (I think it regressed out the effect of genotype and gender, 
> that is it takes the genotype as a covariable)
>
> Or I should create another fsgd file, that has 4 classes: AF, AM, BF, 
> BM. And I can use the matrix (0.5 0.5 -0.5 -0.5 0 0 0 0 0 0 0 0) to 
> perform the main effect of diagnosis.
> Which one of two mentioned matrix is right?
> Thanks a lot.
> Best wishes
> Meng
>
>

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