Hi Anastasia,
I started recently using Tracula to do tractography for my DTI data. I did the 
analysis exactly as mentioned in wiki. The problem is that when I am repeating 
the third step (trac-all -path -c ) for the same subjects to generate the 
statistics; Tracula is updating all the information mentioned in the file 
"pathstats.overal.txt" regarding the tract's volume and the tract's metrics 
values.
Every time the output is different. I think we have a problem in the scripts or 
something like this?

Attached are three "pathstats.overal.txt" files for the same subject after 
three consecutive runs for the "trac-all -path -c" (the same subject)


Looking forward for your support
Thanks
# Title Pathway Statistics
#
# generating_program /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats
# cvs_version 
# cmdline /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats --intrc 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr
 --dtbase 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dmri/dtifit 
--path fmajor --subj 002201 --out 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.overall.txt
 --outvox 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.byvoxel.txt
 
# sysname Linux
# hostname icepuff2
# machine x86_64
# user malshikh
# anatomy_type pathway
#
# subjectname 002201
# pathwayname fmajor
#
Count 1500
Volume 1151
Len_Min 54
Len_Max 91
Len_Avg 71.1467
Len_Center 70
AD_Avg 0.00143148
AD_Avg_Weight 0.00148653
AD_Avg_Center 0.00146087
RD_Avg 0.000475763
RD_Avg_Weight 0.000414811
RD_Avg_Center 0.000390255
MD_Avg 0.000794336
MD_Avg_Weight 0.000772051
MD_Avg_Center 0.000747128
FA_Avg 0.593211
FA_Avg_Weight 0.653136
FA_Avg_Center 0.664371
# Title Pathway Statistics
#
# generating_program /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats
# cvs_version 
# cmdline /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats --intrc 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr
 --dtbase 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dmri/dtifit 
--path fmajor --subj 002201 --out 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.overall.txt
 --outvox 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.byvoxel.txt
 
# sysname Linux
# hostname icepuff2
# machine x86_64
# user malshikh
# anatomy_type pathway
#
# subjectname 002201
# pathwayname fmajor
#
Count 1500
Volume 998
Len_Min 55
Len_Max 105
Len_Avg 70.452
Len_Center 70
AD_Avg 0.00144797
AD_Avg_Weight 0.00148739
AD_Avg_Center 0.00147972
RD_Avg 0.000450739
RD_Avg_Weight 0.000414348
RD_Avg_Center 0.000411816
MD_Avg 0.00078315
MD_Avg_Weight 0.000772027
MD_Avg_Center 0.000767783
FA_Avg 0.614367
FA_Avg_Weight 0.654019
FA_Avg_Center 0.648396
# Title Pathway Statistics
#
# generating_program /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats
# cvs_version 
# cmdline /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats --intrc 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr
 --dtbase 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dmri/dtifit 
--path fmajor --subj 002201 --out 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.overall.txt
 --outvox 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.byvoxel.txt
 
# sysname Linux
# hostname icepuff2
# machine x86_64
# user malshikh
# anatomy_type pathway
#
# subjectname 002201
# pathwayname fmajor
#
Count 1500
Volume 693
Len_Min 56
Len_Max 97
Len_Avg 72.1413
Len_Center 29
AD_Avg 0.00150666
AD_Avg_Weight 0.00151482
AD_Avg_Center 0.00130182
RD_Avg 0.000431594
RD_Avg_Weight 0.000428958
RD_Avg_Center 0.000632432
MD_Avg 0.000789951
MD_Avg_Weight 0.000790911
MD_Avg_Center 0.000855561
FA_Avg 0.648118
FA_Avg_Weight 0.653595
FA_Avg_Center 0.457097
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