Hi,

we have brain images stored as DICOM and as NIFTI files. For some time we used 
to import the NIFTI files to freesurfer for segmentation. I was curious if it 
makes a difference if we would import the DICOM files directly, and was 
surprised that it actually did. Here are the commands we used:

# Import DICOM files
recon-all -s 3003PC_dcm -i 
../../Daten/DICOM/3003PC/M_MPRAGE_sag/IM-0010-0001.dcm

# Convert DICOM to NIFTI and import NIFTI file
mri_convert ../../Daten/DICOM/3003PC/M_MPRAGE_sag/IM-0010-0001.dcm 3003PC.nii.gz
recon-all -s 3003PC_nii -i 3003PC.nii.gz

# Segmentation of DICOM file import
recon-all -s 3003PC_dcm -all

# Segmentation of NIFTI file import
recon-all -s 3003PC_nii -all

Here are the first 3 volumes from aseg.stats for 3003PC_dcm (DICOM import)

  1   4      5612     5611.8  Left-Lateral-Ventricle            27.6355    
12.8465     8.0000    93.0000    85.0000 
  2   5       452      452.2  Left-Inf-Lat-Vent                 42.1100    
14.9377     7.0000    86.0000    79.0000 
  3   7     13765    13765.1  Left-Cerebellum-White-Matter      87.7808     
6.6008    36.0000   109.0000    73.0000 

and here the the first 3 volumes for 3003PC_nii (NIFTI import)

  1   4      5582     5582.2  Left-Lateral-Ventricle            27.4507    
12.7212     8.0000    93.0000    85.0000 
  2   5       421      421.1  Left-Inf-Lat-Vent                 40.8032    
14.6554     7.0000    83.0000    76.0000 
  3   7     13899    13898.6  Left-Cerebellum-White-Matter      87.6707     
6.7539    36.0000   109.0000    73.0000 

The differences are small but I think there should be no differences at all. 
Has someone an explanation for this differences and which file format is better?

Greetings
Peter

P. S.: Our system: freesurfer-Linux-centos6_x86_64-stable-pub-v5.3.0 on Ubuntu 
14.04 
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