Hello FreeSurfer Community,

I am attempting to use mri_watershed to create BEM surfaces, but for some of my 
subjects part of the cerebellum is being cut off.  Adjusting the threshold was 
not sufficient, so I attempted to add a seed point in the cerebellum using the 
following command:

mri_watershed -atlas -useSRAS -surf ./surfout -s [105 182 81] ./T1.mgz 
./brainvol

The coordinates for the seed point are from the T1.mgz image.  I reran 
mri_watershed and the seed point does not seem to be taken into consideration 
so I'm thinking that the input format I used may be wrong.  Could someone 
please tell me if I am not using the proper input format?  And what format to 
use if I wish to add multiple seed points?  Command output is attached.

Thanks in advance!

Kristen


Kristen Kanoff
Clinical Research Coordinator II
Department of Neurology
Massachusetts General Hospital
Boston, MA
Ph: 617-726-3958
osboxes@osboxes:~/Documents/freesurfer/subjects/iDBS002/mri$ mri_watershed 
-atlas -useSRAS -surf ./surfout -s [105 182 81] ./T1.mgz ./brainvol

Mode:          Atlas analysis
Mode:          use surfaceRAS to save surface vertex positions
Mode:          Saving out BEM surfaces

*********************************************************
The input file is ./T1.mgz
The output file is ./brainvol

*************************WATERSHED**************************
Sorting...
      T1-weighted MRI image
      modification of the preflooding height to 15 percent
      Count how many 110 voxels are present :  176432

      Find the largest 110-component...
             heap usage = 336340 Kbytes.
             current max segment has 154566 voxels
             removing small segments (less than 1 percent of maxarea).
             heap usage = 130864 Kbytes.
             removing small segments (less than 1 percent of maxarea).done
      And identify it as the main brain basin...done
      Main component: 154566 voxels
      first estimation of the COG coord: x=128 y=122 z=94 r=81
      first estimation of the main basin volume: 2241917 voxels
      global maximum in x=146, y=114, z=55, Imax=255
      CSF=24, WM_intensity=110, WM_VARIANCE=5
      WM_MIN=110, WM_HALF_MIN=110, WM_HALF_MAX=110, WM_MAX=110 
      preflooding height equal to 15 percent
done.
Analyze...

      main basin size=34171332 voxels, voxel volume =1.000 
                     = 34171332 mmm3 = 34171.332 cm3
done.
PostAnalyze...
      ambiguous basin, merged: at least 12 ambiguous voxels; size: 1490 voxels
      ambiguous basin, merged: at least 4 ambiguous voxels; size: 60 voxels
      ambiguous basin, merged: at least 11 ambiguous voxels; size: 1280 voxels
      ambiguous basin, merged: at least 7 ambiguous voxels; size: 260 voxels
      ambiguous basin, merged: at least 10 ambiguous voxels; size: 913 voxels
      ambiguous basin, merged: at least 7 ambiguous voxels; size: 307 voxels
      ***** 6 basin(s) merged in 4 iteration(s)
      ***** 4310 voxel(s) added to the main basin
      ambiguous basin, non merged: 6 ambiguous voxels; size: 2335 voxels
done.

****************TEMPLATE DEFORMATION****************

      second estimation of the COG coord: x=128,y=120, z=92, r=12891 iterations
^^^^^^^^ couldn't find WM with original limits - expanding ^^^^^^

   GLOBAL      CSF_MIN=1, CSF_intensity=2, CSF_MAX=31 , nb = 43064
 Problem with the least square interpolation in GM_MIN calculation.
   
                     CSF_MAX  TRANSITION  GM_MIN  GM
    GLOBAL     
  before analyzing :    31,      29,        28,   54
  after  analyzing :    20,      29,        29,   35
      mri_strip_skull: done peeling brain
      highly tesselated surface with 10242 vertices
      matching...59 iterations

*********************VALIDATION*********************
curvature mean = -0.013, std = 0.011
curvature mean = 67.196, std = 9.490

Rigid alignment...
      scanning 32.00 degree nbhd, min sse =  6.39 at ( 0.00,  8.00,  8.00)
      scanning 16.00 degree nbhd, min sse =  6.10 at ( 0.00, -4.00, -4.00)
      scanning  8.00 degree nbhd, min sse =  6.10 at ( 0.00,  0.00,  0.00)
      scanning  4.00 degree nbhd, min sse =  6.08 at ( 1.00,  1.00,  1.00)
done
      before rotation: sse = 6.68, sigma = 13.72
      after  rotation: sse = 6.08, sigma = 10.80
Localization of inacurate regions: Erosion-Dilation steps
      the sse mean is  7.43, its var is 10.75   
      before Erosion-Dilatation  6.23% of inacurate vertices
      after  Erosion-Dilatation  9.18% of inacurate vertices
      Validation of the shape of the surface done.
Scaling of atlas fields onto current surface fields

********FINAL ITERATIVE TEMPLATE DEFORMATION********
Compute Local values csf/gray
Fine Segmentation...40 iterations

      mri_strip_skull: done peeling brain

Brain Size = 1413043 voxels, voxel volume = 1.000 mm3
           = 1413043 mmm3 = 1413.043 cm3

      outer skin surface matching...187 iterations

******************************
Saving ./brainvol
done
mri_watershed utimesec    513.928000
mri_watershed stimesec    0.340000
mri_watershed ru_maxrss   370808
mri_watershed ru_ixrss    0
mri_watershed ru_idrss    0
mri_watershed ru_isrss    0
mri_watershed ru_minflt   172015
mri_watershed ru_majflt   0
mri_watershed ru_nswap    0
mri_watershed ru_inblock  0
mri_watershed ru_oublock  35688
mri_watershed ru_msgsnd   0
mri_watershed ru_msgrcv   0
mri_watershed ru_nsignals 0
mri_watershed ru_nvcsw    4152
mri_watershed ru_nivcsw   5763
mri_watershed done
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