Hello FreeSurfer Community, I am attempting to use mri_watershed to create BEM surfaces, but for some of my subjects part of the cerebellum is being cut off. Adjusting the threshold was not sufficient, so I attempted to add a seed point in the cerebellum using the following command:
mri_watershed -atlas -useSRAS -surf ./surfout -s [105 182 81] ./T1.mgz ./brainvol The coordinates for the seed point are from the T1.mgz image. I reran mri_watershed and the seed point does not seem to be taken into consideration so I'm thinking that the input format I used may be wrong. Could someone please tell me if I am not using the proper input format? And what format to use if I wish to add multiple seed points? Command output is attached. Thanks in advance! Kristen Kristen Kanoff Clinical Research Coordinator II Department of Neurology Massachusetts General Hospital Boston, MA Ph: 617-726-3958
osboxes@osboxes:~/Documents/freesurfer/subjects/iDBS002/mri$ mri_watershed -atlas -useSRAS -surf ./surfout -s [105 182 81] ./T1.mgz ./brainvol Mode: Atlas analysis Mode: use surfaceRAS to save surface vertex positions Mode: Saving out BEM surfaces ********************************************************* The input file is ./T1.mgz The output file is ./brainvol *************************WATERSHED************************** Sorting... T1-weighted MRI image modification of the preflooding height to 15 percent Count how many 110 voxels are present : 176432 Find the largest 110-component... heap usage = 336340 Kbytes. current max segment has 154566 voxels removing small segments (less than 1 percent of maxarea). heap usage = 130864 Kbytes. removing small segments (less than 1 percent of maxarea).done And identify it as the main brain basin...done Main component: 154566 voxels first estimation of the COG coord: x=128 y=122 z=94 r=81 first estimation of the main basin volume: 2241917 voxels global maximum in x=146, y=114, z=55, Imax=255 CSF=24, WM_intensity=110, WM_VARIANCE=5 WM_MIN=110, WM_HALF_MIN=110, WM_HALF_MAX=110, WM_MAX=110 preflooding height equal to 15 percent done. Analyze... main basin size=34171332 voxels, voxel volume =1.000 = 34171332 mmm3 = 34171.332 cm3 done. PostAnalyze... ambiguous basin, merged: at least 12 ambiguous voxels; size: 1490 voxels ambiguous basin, merged: at least 4 ambiguous voxels; size: 60 voxels ambiguous basin, merged: at least 11 ambiguous voxels; size: 1280 voxels ambiguous basin, merged: at least 7 ambiguous voxels; size: 260 voxels ambiguous basin, merged: at least 10 ambiguous voxels; size: 913 voxels ambiguous basin, merged: at least 7 ambiguous voxels; size: 307 voxels ***** 6 basin(s) merged in 4 iteration(s) ***** 4310 voxel(s) added to the main basin ambiguous basin, non merged: 6 ambiguous voxels; size: 2335 voxels done. ****************TEMPLATE DEFORMATION**************** second estimation of the COG coord: x=128,y=120, z=92, r=12891 iterations ^^^^^^^^ couldn't find WM with original limits - expanding ^^^^^^ GLOBAL CSF_MIN=1, CSF_intensity=2, CSF_MAX=31 , nb = 43064 Problem with the least square interpolation in GM_MIN calculation. CSF_MAX TRANSITION GM_MIN GM GLOBAL before analyzing : 31, 29, 28, 54 after analyzing : 20, 29, 29, 35 mri_strip_skull: done peeling brain highly tesselated surface with 10242 vertices matching...59 iterations *********************VALIDATION********************* curvature mean = -0.013, std = 0.011 curvature mean = 67.196, std = 9.490 Rigid alignment... scanning 32.00 degree nbhd, min sse = 6.39 at ( 0.00, 8.00, 8.00) scanning 16.00 degree nbhd, min sse = 6.10 at ( 0.00, -4.00, -4.00) scanning 8.00 degree nbhd, min sse = 6.10 at ( 0.00, 0.00, 0.00) scanning 4.00 degree nbhd, min sse = 6.08 at ( 1.00, 1.00, 1.00) done before rotation: sse = 6.68, sigma = 13.72 after rotation: sse = 6.08, sigma = 10.80 Localization of inacurate regions: Erosion-Dilation steps the sse mean is 7.43, its var is 10.75 before Erosion-Dilatation 6.23% of inacurate vertices after Erosion-Dilatation 9.18% of inacurate vertices Validation of the shape of the surface done. Scaling of atlas fields onto current surface fields ********FINAL ITERATIVE TEMPLATE DEFORMATION******** Compute Local values csf/gray Fine Segmentation...40 iterations mri_strip_skull: done peeling brain Brain Size = 1413043 voxels, voxel volume = 1.000 mm3 = 1413043 mmm3 = 1413.043 cm3 outer skin surface matching...187 iterations ****************************** Saving ./brainvol done mri_watershed utimesec 513.928000 mri_watershed stimesec 0.340000 mri_watershed ru_maxrss 370808 mri_watershed ru_ixrss 0 mri_watershed ru_idrss 0 mri_watershed ru_isrss 0 mri_watershed ru_minflt 172015 mri_watershed ru_majflt 0 mri_watershed ru_nswap 0 mri_watershed ru_inblock 0 mri_watershed ru_oublock 35688 mri_watershed ru_msgsnd 0 mri_watershed ru_msgrcv 0 mri_watershed ru_nsignals 0 mri_watershed ru_nvcsw 4152 mri_watershed ru_nivcsw 5763 mri_watershed done
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