what do you mean?

On 5/31/19 3:00 PM, Winkelbeiner, Stephanie A wrote:
>
>         External Email - Use Caution
>
> Hi Freesurfers,
>
> the number of my parcels increases after extending them into the WM with
>
> mri_aparc2aseg \
>     --s s001 \
>     --annot 500.aparc \
>     --wmparc-dmax 2 \
>     --labelwm \
>     --hypo-as-wm \
>     --o mri/aparc.500+2mm.nii.gz
>
> Could that really be the case? And if so, what can I do to keep the 
> original number of parcels?
> (See more detailed description of my approach below)
>
> I would be very grateful for some suggestions!
> Thanks a lot,
> Stephanie Winkelbeiner
>
> -----------------------------------------------
> *Stephanie Winkelbeiner, PhD*
>
> Psychiatry Research
>
> Zucker Hillside Hospital
>
> 75-59 263 Street
>
> Glen Oaks, NY 11004
>
> Tel: (718) 470-8267
>
> Email:swinkel...@northwell.edu <mailto:swinkel...@northwell.edu>
>
>
>
> ------------------------------------------------------------------------
> *From:* freesurfer-boun...@nmr.mgh.harvard.edu 
> <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Winkelbeiner, 
> Stephanie A <swinkel...@northwell.edu>
> *Sent:* Friday, 24 May 2019 6:08 PM
> *To:* Freesurfer support list
> *Subject:* Re: [Freesurfer] [EXTERNAL] Re: Checking number of parcels 
> after transformation of an annotation file into a .nii.gz file
>
> */External Email. Use Caution./*
>
>         External Email - Use Caution
>
> Hi Doug,
>
> thanks again for the great tip with MRIread - worked perfectly (after 
> gunzipping the parc300.nii.gz).
>
> So, this means I have the next problem now: instead of 300 parcels 
> this image contains 638 parcels...
>
> How I created parc300.nii.gz:
>
>  1. MATLAB script to create annotation file with 150 parcels/hemisphere
>  2. extending the parcels 2mm into the WM with mri_aparc2aseg (see 1st
>     email)
>  3. coregistering to the T1 to get the transformation matrix (bbregister)
>  4. coregistering to DWI space (mri_vol2vol)
>
> I found in an earlier answer of yours that the extention into the WM 
> might duplicate the parcels/labels (*MailScanner has detected a 
> possible fraud attempt from "urldefense.proofpoint.com" claiming to 
> be* 
> https://mail.nmr.mgh.harvard.edu/pipermail//freesurfer/2018-February/055952.html
>  
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_pipermail__freesurfer_2018-2DFebruary_055952.html&d=DwMFAw&c=vq5m7Kktb9l80A_wDJ5D-g&r=ycrYUdKGMCyTnsIy5TvOdBwS8HE5ZtmVMesLULEN8xE&m=O04ejT4OMCoABZuHtxcNBFTV5kd2I3iNoEfTbDuX5Q8&s=JeMKgrHrp_mkwc0hI-lKcpr8YPLuQKG9Cqg1-e73Cds&e=>)
> Following your suggestions in that email exchange, I did the following:
> ## 1. Create a mask of the wm labels:
> mri_binarize \
>     --i aparc.500+2mm.nii.gz \
>     --min 2999.5 \
>     --max 4035.5 \
>     --o wm.mask.500.nii.gz
>
> ## 2. Mask the wmparc:
> mri_mask \
>     aparc.500+2mm.nii.gz \
>     wm.mask.500.nii.gz \
>     aparc.500+2mm.masked.nii.gz
>
> This results in only two parcels... What am I doing wrong or 
> overlooking here?
>
> Thanks a lot for your help (as always)!
> Cheers,
> Stephanie
>
>
> ------------------------------------------------------------------------
> *From:* freesurfer-boun...@nmr.mgh.harvard.edu 
> <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Winkelbeiner, 
> Stephanie A <swinkel...@northwell.edu>
> *Sent:* Wednesday, 22 May 2019 6:23 PM
> *To:* Freesurfer support list
> *Subject:* Re: [Freesurfer] [EXTERNAL] Re: Checking number of parcels 
> after transformation of an annotation file into a .nii.gz file
>
> */External Email. Use Caution./*
>
>         External Email - Use Caution
>
> That sounds  like a perfect plan!
>
> I gonna try that immediately! Thanks so much, Doug!
> ------------------------------------------------------------------------
> *From:* freesurfer-boun...@nmr.mgh.harvard.edu 
> <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Greve, Douglas 
> N.,Ph.D. <dgr...@mgh.harvard.edu>
> *Sent:* Wednesday, 22 May 2019 6:19 PM
> *To:* freesurfer@nmr.mgh.harvard.edu
> *Subject:* [EXTERNAL] Re: [Freesurfer] Checking number of parcels 
> after transformation of an annotation file into a .nii.gz file
> External Email. Use Caution.
>
> the way that pops to mind is to use matlab
> a = MRIread('aparc.250+2mm.nii.gz');
>
> u = unique(a.vol(:));
> length(u) will give you the number of unique segmentation number
> (including 0)
>
> On 5/22/19 5:54 PM, Winkelbeiner, Stephanie A wrote:
> >
> >         External Email - Use Caution
> >
> > Hi Freesurfers,
> >
> > I'm struggling to find out if the number of parcels during a process
> > of transformations from annot -> nii.gz has changed.
> >
> > I extended the parcels from 500.annot 2mm into the WM with
> > mri_aparc2aseg \
> > --s s001 \
> > --annot 500.aparc \
> > --wmparc-dmax 2 \
> > --labelwm \
> > --hypo-as-wm \
> > --o mri/aparc.250+2mm.nii.gz
> > and coregistered the resulting file to the DWI image using bbregister
> > and mri_vol2vol.
> >
> > Using afni's probilistic tractography pipeline, I end up with more
> > parcels than I thought I had.
> >
> > Is there a way to check the number of parcels after extension into the
> > WM (mri_aparc2aseg)?
> >
> > I would be extremely grateful for some insight!
> > Thanks,
> > Stephanie
> >
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> delete the original communication and any attachment from any 
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> entity to whom or to which it is addressed, and may contain 
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