Hi Karel,

These are the commands we would run to calculate thickness in an ROI volume:

mri_vol2surf
mri_vol2label (please ignore misleading name, will also work on surfaces)
mris_anatomicalstats


The wiki has documentation on all of these programs, but if you would like 
further details feel free to reach out!
https://surfer.nmr.mgh.harvard.edu/fswiki
-Matt

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