Hi Ken, 

Hmm, not sure. Does it give a reason why it cannot be loaded? Could be read 
permissions, could be corrupted. 

Generally the commands look good. You could also try to check the subject SPC 
on the base-template surface. Those files should also be there in the base dir. 

Best, Martin


> On 31. Aug 2020, at 04:02, KennethSPrice <kennethspr...@protonmail.com> wrote:
> 
> 
> 
> 
> Hello Freesurfers!
> 
> I am working through the longitudinal tutorial found here:
> https://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/LongitudinalTutorial 
> <https://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/LongitudinalTutorial>
> 
> To keep it simple, I ran longitudinal processing on 2 timepoints for one 
> subject. My qdec file had only two entries. I would like to look at pct 
> difference in thickness for one subject. I ran
> 
> long_mris_slopes --qdec qdec.dat --meas thickness --hemi lh --do-avg 
> --do-rate --do-pc1 --do-spc --do-stack --do-label --time years --qcache 
> fsaverage --sd $SUBJECTS_DIR
> 
> When I run the overlay I get an error saying that "the overlay cannot be 
> loaded", although the file exists.  
> 
> freeview -f 
> $FREESURFER_HOME/subjects/fsaverage/surf/lh.pial:overlay=$SUBJECTS_DIR/OAS2_0001/surf/lh.long.thickness-spc.fwhm15.fsaverage.mgh:overlay_threshold=2,5
> 
> First of all, is this method sound in order to visualize pct change in 
> thickness for one subject? If so, why is the overlay not loading?
> 
> Best,
> Ken
> 
> 
> Sent with ProtonMail <https://protonmail.com/> Secure Email.
> 
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