In your mkanalysis command, why are you specifying the mask and the funcstem? These are tricky options to get right ...

On 11/29/2021 9:50 AM, Hiersche, Kelly J. wrote:

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Hello FreeSurfer Developers,

First, I am new to using Freesurfer, so I apologize if any information is unclear.

I am attempting to use the following tutorial *MailScanner has detected a possible fraud attempt from "secure-web.cisco.com" claiming to be* https://surfer.nmr.mgh.harvard.edu/fswiki/FsFastTutorialV5.1/FsFastFirstLevel <https://secure-web.cisco.com/1i0LcvXc_pWxYrG0y8LzlZO7IfvmC_UpV6cd9EBpLiYz691LC8ZZxenFAFLThZDwWA9B3gqr427fuo4gIW_UApRZ4HnhyE--4n1riYpxVkN51_HSluoN7RvhScv1DHFw7bSCJ0zsqXSx9101qhpJCaDVO6iIRiJSWf539hQDEXUv0GRQj5G5pJxWWZkHuaNaJjK8tH-3_N09V9VHNplUH_00QltaR2DYkF533lkMSxJ_21ufiyfdkpYimByzPbHE4bVWAWNZ0Vy4A0e4ZiEjRKLjfrLejQ08R-VuM6jxUMIKn7IgT-ICmWdfQrIOzL_2vYY4e9sMGddb5z3vdRMj_XA/https%3A%2F%2Fsurfer.nmr.mgh.harvard.edu%2Ffswiki%2FFsFastTutorialV5.1%2FFsFastFirstLevel> to do a surface based GLM in an individual subject (using my own data, not the tutorial data), but I am getting the following error:

Error using MRIread (line 76)
ERROR: cannot determine format of
/*subnum/bold/runnum/masks*/fmcpr.brainmask.fsaverage.lh
(MRIread)

I am getting that error because the fmcpr.brainmask.fsaverage.lh does not exist. To fix this error, I re-ran preprocessing using the following tutorial: *MailScanner has detected a possible fraud attempt from "secure-web.cisco.com" claiming to be* https://surfer.nmr.mgh.harvard.edu/fswiki/FsFastTutorialV5.1/FsFastPreProc <https://secure-web.cisco.com/1Sor7-h_gxGA_NBXCIF2cnCakBuzWn-uVbkP71hrF-15-uN-yPZMZmi3kEC7J04gY__hv7B9nXr3WBKeUKxOm2ARABA3qbbXeq_UCQP6kYpAlwRN1Lmjs4LDQzWIvmqwpbuLNUdAopRjQRoR0TIdvr8VV-G4exusT4txcHYaHojYOsyNUW_3md9vLAoF0m-ujmr_Rr0EuurR5bAmw6rwn87kGNQPpVJJ52hOxpUHpxX63dkNnzfH2OOcXR3Js60fj98Rub3VqUXKmU9KYBQAVgOKng5ytmCjF1v6PYw5mROgvvlEAXnT5DXrqtnaNluG0owlDYiSfrY-MTokGuBeLCQ/https%3A%2F%2Fsurfer.nmr.mgh.harvard.edu%2Ffswiki%2FFsFastTutorialV5.1%2FFsFastPreProc>

After preprocessing, I still got the same error.

*Here are the files that do exist in that path: *subnum/bold/run-num/masks* * brain.e3.nii.gz            brain.fsaverage.lh.pr.nii.gz  brain.fsaverage.rh.pr.nii.gz  brain.mni305.2mm.pr.nii.gz  brain.nii.gz.mkbrainmask.log brain.fsaverage.lh.nii.gz  brain.fsaverage.rh.nii.gz     brain.mni305.2mm.nii.gz brain.nii.gz

*There are some fmcpr files in this folder: subnum/bold/run-num*
fmcpr.brainmask.norm.2x2x2.nii.gz     fmcpr.mcdat fmcpr.norm.nii.gz              fmcpr.sm5.fsaverage.rh.nii.gz fmcpr.brainmask.normtmp.2x2x2.nii.gz  fmcpr.nii.gz        fmcpr.sm0.norm.nii.gz  fmcpr.sm5.mni305.2mm.nii.gz fmcpr.mat.aff12.1D  fmcpr.nii.gz.mclog  fmcpr.sm5.fsaverage.lh.nii.gz  fmcpr.sm5.norm.nii.gz


*Here are the commands I am running:*
*mkanalysis-sess*-fsd bold -rlf $analysis_stem-$run_set.rlf -surface fsaverage $hemi -event-related -paradigm $PARA_NAME -refeventdur 18 -nconditions $nconditions -mask $func_stem.brainmask -gammafit 2.25 1.25 -nuisreg mcprextreg 6 -TR 1.0000 -polyfit 1 -analysis $analysis_stem-$run_set-$func_stem-$tpe.sm5.$hemi.$REG_STEM -tpexclude $tpe$TPE_STEM -funcstem $func_stem.sm$kernel$REG_STEM -per-run

*mkcontrast-sess*-analysis $analysis_stem-$run_set-$func_stem-$tpe.sm5.$hemi.$REG_STEM -contrast Con1-Con2 -a 1 -c 2

*preproc-sess*-fsd bold -rlf $analysis_stem-$run_set.rlf -surface fsaverage lhrh -mni305 -fwhm 5 -per-run

 1. I am running freesurfer through matlab on the Ohio Supercomputer;
    freesurfer/6.0.0

Thank you for all your troubleshooting help!

Kelly

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