You can also look in pctsurfcon. This compute the percent gray/white contrast. It does so on the T1w, but you could look inside the script to see how it does it and adapt to your situation.

On 2/23/2022 3:26 PM, Adam Martersteck wrote:

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Hi Dylan,

Give this a try:
fscalc gm_lh.nii.gz div wm_lh.nii.gz --o gmwmratio_lh.nii.gz

On Wed, Feb 23, 2022 at 12:24 PM Tisdall, Dylan <mtisd...@pennmedicine.upenn.edu> wrote:

          External Email - Use Caution

    Hi all,

    I've generated two surface overlays, using mri_vol2surf with
    positive and negative projfrac to get a GM and WM samples
    (gm_lh.nii.gz and wm_lh.nii.gz). I'd now like to take the
    vertex-wise ratio of these two overlays, and make a new one
    (gmwmratio_lh.nii.gz). I've tried doing this as:

    fslmaths gm_lh.nii.gz -div wm_lh.nii.gz gmwmratio_lh.nii.gz

    but the result is:

    Image Exception: #22 :: Failed to read volume gm_lh.nii.gz
    Error : Error: Nonsensical ROI for gm_lh.nii.gz

    Is there another tool I can use to compute this ratio? Is there a
    better workflow than making two surfaces and dividing?


    Thanks,
    Dylan



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