yes, it does

On 2/25/2022 5:14 PM, Jennifer Bramen wrote:

        External Email - Use Caution

Hi Doug

I want to confirm that the signal any region flagged as cortex is restricted to gray matter. If that is the case, then I finally have what I need. Thank you.

Warm regards,


Jennifer Bramen, PhD
(she/her/hers)
Senior Research Scientist, Neuroimaging
Assistant Professor
Pacific Brain Health Center | Pacific Neuroscience Institute & Foundation | Providence Saint John's Health Center
1301 20th St. #150 Santa Monica, CA 90404
*Phone:* 310-525-0865  | *Fax: *310-315-4069

*From: *<freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of "Douglas N. Greve" <dgr...@mgh.harvard.edu>
*Reply-To: *Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
*Date: *Friday, February 25, 2022 at 10:25 AM
*To: *"freesurfer@nmr.mgh.harvard.edu" <freesurfer@nmr.mgh.harvard.edu>
*Subject: *Re: [Freesurfer] PetSurfer: subcortical volume-based analysis

Are you doing an ROI analysis or a voxel-wise analysis? If an ROI analysis, then use mri_gtmpvc (with or without PVC). This will give you GM, WM, and CSF ROIs. Or do you mean you want a single average across all GM?

On 2/24/2022 1:15 PM, Jennifer Bramen wrote:

    *        External Email - Use Caution *

    I would like to measure uptake exclusively in gray matter. What
    would you recommend to accomplish this?

    In my previous message, I was wondering if adding a gray matter
    mask would be a potential way to restrict the analysis to gray
    matter. Thanks!

    Warm regards,


    Jennifer Bramen, PhD
    (she/her/hers)
    Senior Research Scientist, Neuroimaging
    Assistant Professor
    Pacific Brain Health Center | Pacific Neuroscience Institute &
    Foundation | Providence Saint John's Health Center
    1301 20th St. #150 Santa Monica, CA 90404
    *Phone:* 310-525-0865  | *Fax: *310-315-4069

    *From: *<freesurfer-boun...@nmr.mgh.harvard.edu>
    <mailto:freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of
    "Douglas N. Greve" <dgr...@mgh.harvard.edu>
    <mailto:dgr...@mgh.harvard.edu>
    *Reply-To: *Freesurfer support list
    <freesurfer@nmr.mgh.harvard.edu>
    <mailto:freesurfer@nmr.mgh.harvard.edu>
    *Date: *Tuesday, February 22, 2022 at 3:00 PM
    *To: *"freesurfer@nmr.mgh.harvard.edu"
    <mailto:freesurfer@nmr.mgh.harvard.edu>
    <freesurfer@nmr.mgh.harvard.edu>
    <mailto:freesurfer@nmr.mgh.harvard.edu>
    *Subject: *Re: [Freesurfer] PetSurfer: subcortical volume-based
    analysis

    I don't understand. What mask?

    On 2/22/2022 8:55 AM, Jennifer Bramen wrote:

        *        External Email - Use Caution *

        If I only want to extract intensity from gray matter, do I
        need to add an additional mask?

        Thanks!

        Jennifer

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        ------------------------------------------------------------------------

        *From:* freesurfer-boun...@nmr.mgh.harvard.edu
        <freesurfer-boun...@nmr.mgh.harvard.edu>
        <mailto:freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of
        Douglas N. Greve <dgr...@mgh.harvard.edu>
        <mailto:dgr...@mgh.harvard.edu>
        *Sent:* Monday, February 21, 2022 7:13:39 PM
        *To:* freesurfer@nmr.mgh.harvard.edu
        <freesurfer@nmr.mgh.harvard.edu>
        <mailto:freesurfer@nmr.mgh.harvard.edu>
        *Subject:* Re: [Freesurfer] PetSurfer: subcortical
        volume-based analysis

        The GTM gives you values for each ROI regardless of whether it
        is GM, WM, or CSF. They will probably give you similar
        answers, but the GTM will be more robust.

        On 2/21/2022 8:48 PM, Jennifer Bramen wrote:

            *        External Email - Use Caution *

            Thanks! Yes, I already mapped my PET to anatomical space
            using mri_vol2vol and glmdir/aux/bbpet2anat.lta.

            RE: selection of mgx instead of gtm: I am analyzing
            amyloid PET data and I believe it is recommended to
            extract gray matter localized PET data. Is that what I am
            doing by using the mgx image?

            Warm regards,


            Jennifer Bramen, PhD
            (she/her/hers)
            Senior Research Scientist, Neuroimaging
            Assistant Professor
            Pacific Brain Health Center | Pacific Neuroscience
            Institute & Foundation | Providence Saint John's Health
            Center
            1301 20th St. #150 Santa Monica, CA 90404
            *Phone:* 310-525-0865  | *Fax: *310-315-4069

            *From: *<freesurfer-boun...@nmr.mgh.harvard.edu>
            <mailto:freesurfer-boun...@nmr.mgh.harvard.edu> on behalf
            of "Douglas N. Greve" <dgr...@mgh.harvard.edu>
            <mailto:dgr...@mgh.harvard.edu>
            *Reply-To: *Freesurfer support list
            <freesurfer@nmr.mgh.harvard.edu>
            <mailto:freesurfer@nmr.mgh.harvard.edu>
            *Date: *Monday, February 21, 2022 at 2:02 PM
            *To: *"freesurfer@nmr.mgh.harvard.edu"
            <mailto:freesurfer@nmr.mgh.harvard.edu>
            <freesurfer@nmr.mgh.harvard.edu>
            <mailto:freesurfer@nmr.mgh.harvard.edu>
            *Subject: *Re: [Freesurfer] PetSurfer: subcortical
            volume-based analysis

            You need to first map your pet to the anatomical space
            using mri_vol2vol and the registration
            glmdir/aux/bbpet2anat.lta
            BTW, why are you computing the ROI anlaysis from the mgx
            data instead of using the output from the gtm? Note that
            you cannot use the white matter values that come from  mgx.

            On 2/21/2022 12:57 PM, Jennifer Bramen wrote:

                *        External Email - Use Caution *

                I am trying to extract mean intensity in my PET data
                for every ROI. Doug said to run mri_segstats passing
                the PET with --i and using --seg to spec the
                segmentation. *I would like to confirm that I am using
                this properly.*

                mri_segstats --i
                $PET_DIR/$ID/gtmpvc.output/mgx.gm_average.T1.nii.gz
                --seg $SUBJECTS_DIR/$ID/mri/aparc+aseg.mgz --annot lh
                --slabel lh --sum
                $PET_DIR/$ID/gtmpvc.output/lh.PET_aparc.aseg

                where
                $PET_DIR/$ID/gtmpvc.output/mgx.gm_average.T1.nii.gz is
                the PET data registered and resampled into the
                individual’s T1-weighrd MRI space.

                Warm regards,


                Jennifer Bramen, PhD
                (she/her/hers)
                Senior Research Scientist, Neuroimaging
                Assistant Professor
                Pacific Brain Health Center | Pacific Neuroscience
                Institute & Foundation | Providence Saint John's
                Health Center
                1301 20th St. #150 Santa Monica, CA 90404
                *Phone:* 310-525-0865  | *Fax: *310-315-4069

                *From: *<freesurfer-boun...@nmr.mgh.harvard.edu>
                <mailto:freesurfer-boun...@nmr.mgh.harvard.edu> on
                behalf of "Douglas N. Greve" <dgr...@mgh.harvard.edu>
                <mailto:dgr...@mgh.harvard.edu>
                *Reply-To: *Freesurfer support list
                <freesurfer@nmr.mgh.harvard.edu>
                <mailto:freesurfer@nmr.mgh.harvard.edu>
                *Date: *Wednesday, February 16, 2022 at 7:30 AM
                *To: *"freesurfer@nmr.mgh.harvard.edu"
                <mailto:freesurfer@nmr.mgh.harvard.edu>
                <freesurfer@nmr.mgh.harvard.edu>
                <mailto:freesurfer@nmr.mgh.harvard.edu>
                *Subject: *Re: [Freesurfer] PetSurfer: subcortical
                volume-based analysis

                If you want to do an ROI analysis using FS-based ROIs,
                you should do that at the individual level. Resample
                the PET to the anatoimical space, then run
                mri_segstats passing the PET with --i and using --seg
                to spec the segmentation. If you really want to do it
                in MNI305 space, then it is the same thing just using
                the MNI305-based (2mm) segmentation

                On 2/14/2022 6:11 PM, Jennifer Bramen wrote:

                    *        External Email - Use Caution *

                    Dear Freesurfer Developers

                    I am doing an ROI--based amyloid PET scan
                    analysis. I have completed all of the steps from
                    the PetSurfer wiki. I now have the preprocessed,
                    averaged, resampled, smoothed PET data in MNI305
                    space which is constrained to the subcortical gray
                    matter mask.

                    *How do I extract mean intensity within all of the
                    subcortical ROI?*

                    Thank you!

                    Warm regards,


                    Jennifer Bramen, PhD
                    (she/her/hers)
                    Senior Research Scientist, Neuroimaging
                    Assistant Professor
                    Pacific Brain Health Center | Pacific Neuroscience
                    Institute & Foundation | Providence Saint John's
                    Health Center
                    1301 20th St. #150 Santa Monica, CA 90404
                    *Phone:* 310-525-0865  | *Fax: *310-315-4069




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