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That is what I´m planning to do.
Actually since some subjects have more than 2 time-points I was thinking that 
calculating slopes rather than pair-diff may be a better option.

Can I use long_mris_slopes with --do-spc (etc…) in my mapped PET images to the 
longitudinal surfaces within each subject? Since qdec long table is required, I 
am unsure how to provide my surface-mapped PET images instead of default (i.e. 
thickness).
Alternatively, do I need to run my own GLMs within subject to compute slopes of 
PET signal?

Much appreciated!

Jesus

From: freesurfer-boun...@nmr.mgh.harvard.edu 
[mailto:freesurfer-boun...@nmr.mgh.harvard.edu] On Behalf Of Adam Martersteck
Sent: Wednesday, September 21, 2022 2:46 PM
To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] [EXTERNAL] Re: Longitudinal PET analysis


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On other thought: You may want to do Doug's suggestion of calculating the 
paired-diff in the longitudinal stream's subject-specific unbiased template 
space of each participant instead of doing it all in fsaverage. Then take the 
resultant file, the symmetrized percent change, or pc1, etc. from the 
subject-specific template space to fsaverage for your group statistics.

On Wed, Sep 21, 2022 at 12:39 PM Gomar, Jesus 
<jgo...@northwell.edu<mailto:jgo...@northwell.edu>> wrote:

        External Email - Use Caution
Thank you Doug, I will follow your suggestion.


From: 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
[mailto:freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>]
 On Behalf Of Douglas N. Greve
Sent: Wednesday, September 21, 2022 9:58 AM
To: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: [EXTERNAL] Re: [Freesurfer] Longitudinal PET analysis


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You may be able to use one of the paired diff options in mris_preproc. Run it 
with --help. I also put some info on it below. QDEC has been deprecated



--paired-diff

After concatenating all the inputs together, create a new output file by
computing paired differences, ie, Input1-Input2, Input3-Input4, etc.
There must be an even number of inputs. The inputs are "pruned" before
taking the difference, meaning that values for all inputs are set to 0
if any individual is 0.

--paired-diff-norm

Same as --paired-diff, but normalizes by average of time points, ie,
(Input1-Input2)/((Input1+Input2)/2).

--paired-diff-norm1

Same as --paired-diff, but normalizes by time point 1, ie,
(Input1-Input2)/Input1.

--paired-diff-norm2

Same as --paired-diff, but normalizes by time point 2, ie,
(Input1-Input2)/Input2.

On 9/20/2022 3:31 PM, Gomar, Jesus wrote:

        External Email - Use Caution
Hello Freesurfer developers,

I am interested in analyzing PET data following a longitudinal design.
I have co-registered each PET image to the closest MRI in time processed 
through the longitudinal stream.
I have sampled each co-registered PET image time-point onto fsaverage surface 
via the individual's subject surface (mri_vol2surf).
From this point:

1.       Is it possible to obtain spc (symmetrized percent change) using 
long_mris_slopes for each subject?

2.       Is there a way to input the sampled surfaces into a qdec file for 
long_mris_slopes command?

1) FreeSurfer version: freesurfer-linux-centos6_x86_64-7.2.0.
2) Platform: Ubuntu 18,04.6 LTS
3) uname -a: Linux ubuntu 5.4.0-122-generic-Ubuntu UTC 2022 x86_64 x86_64 
x86_64 GNU/Linux


Thank you!

Jesus
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