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Good afternoon,


Absolutely. Here’s the explanation of the issue in more detail. I have already 
recon-all’d a subject’s T1W image. Now, I’m trying to use the HCP atlas 
annotation files in fsaverage space (downloaded from 
here<https://secure-web.cisco.com/1RUlbihl_P7o4iECkLXdYd7_A71_OWggWGKTcu9o3hEd1Q-TIbJydYcpaMQ6fo6JrKecYvuag9pRj0svtM-tbZHyzOf--ziIZbRt3KZ-s273-wWXgwuWLz3_j6DIrKSiJB9xDfjO7TXoRlBY6j7PD0gZvue_aciq07dI505bqMEmXZ1uP3F-_aCwFXl914_RjYxLmyD2BW3AIYseqx3VO4-lgJJvB1qOkeqtl9XALsZFZTVmYtg3Zjfia6F0tH82nhvFtLOak0mCYm9rH0ZcvOg/https%3A%2F%2Ffigshare.com%2Farticles%2Fdataset%2FHCP-MMP1_0_projected_on_fsaverage%2F3498446>)
 to ultimately make the individual ROIs defined in this atlas in the 
subject-specific volumetric space. In order to do that, I used 
“mri_annotation2label” to find the label files related to the HCP atlas in the 
fsaverage space and then I used “mri_label2label” to transform each label to 
subject-specific space. Then, I used mris_label2annot to create the annotation 
file in subject-specific space and from there I used mri_aparc2aseg to create a 
volumetric .nii file in which each ROI is labeled with a different number. From 
here I was able to extract each ROI in the subject-specific space.


Even though I was able to create my ROIs of interest at the end, I noticed that 
during the execution of the “mris_label2annot” I got warnings stating that some 
vertices mapped to multiple labels. This indicates that during the conversion 
in the prior step using “mri_label2label”, some vertices were assigned to 
multiple labels, however, I’m not sure how I could have prevented this from 
happening. Here’s the syntax I used for the left hemisphere as an example:


mri_label2label --srcsubject fsaverage --srclabel XYZ1 --trgsubject XYZ2 
--trglabel XYZ3 --regmethod surface --hemi lh


Please let me know if I should have included a certain flag while executing 
“mri_label2label” that could have avoided the label overlaps in the new space.


Thank you,

Negin

________________________________
From: freesurfer-boun...@nmr.mgh.harvard.edu 
<freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Douglas N. Greve 
<dgr...@mgh.harvard.edu>
Sent: Monday, May 8, 2023 10:47 AM
To: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] Question about volumetric atlas ROIs overlap


        External Email - Use Caution

I don't understand what you are referring to. Can you elaborate?

On 5/5/2023 7:53 PM, Nadvar, Negin wrote:

        External Email - Use Caution

Hello,

I have a general question about handing atlas ROIs after applying recon-all on 
a T1W image. Could you please clarify how I can minimize/prevent atlas ROIs 
overlap in volumetric subject-specific space when going from surface-based 
space to volumetric space?

Please let me know if you need more information.

Thanks in advance,
Negin




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