External Email - Use Caution        

Hi Doug & Gaurav,

There are some instructions for running recon-all on skull stripped brains in 
the FAQ’s on the wiki
https://surfer.nmr.mgh.harvard.edu/fswiki/UserContributions/FAQ#Q.Ihavealreadyskull-strippeddata.CanIsubmitittorecon-all.3F

pasted below for reference.

Best wishes,
Henry

Q. I have already skull-stripped data. Can I submit it to recon-all?
A: If your skull-stripped volume does not have the cerebellum, then no. If it 
does, then yes, however you will have to run the data a bit differently.
First you must run only -autorecon1 like this:
recon-all -autorecon1 -noskullstrip -s <subjid>

Then you will have to make a symbolic link or copy T1.mgz to brainmask.auto.mgz 
and a link from brainmask.auto.mgz to brainmask.mgz. Finally, open this 
brainmask.mgz file and check that it looks okay (there is no skull, cerebellum 
is intact; use the sample subject bert that comes with your FreeSurfer 
installation to make sure it looks comparable). From there you can run the 
final stages of recon-all:
recon-all -autrecon2 -autorecon3 -s <subjid>


From: freesurfer-boun...@nmr.mgh.harvard.edu 
<freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Douglas N. Greve 
<dgr...@mgh.harvard.edu>
Date: Wednesday, 26 June 2024 at 23:36
To: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] MRI watershed error

⚠ Caution: External sender

not sure, it might work
On 6/26/2024 2:21 PM, Gaurav Ambwani wrote:

        External Email - Use Caution
Dear Freesurfer team,

In short I just wanted to know if it would be possible for me to put a skull 
stripped MRI into freesurfer for recon-all?

Thank you,
Best Wishes,
Gaurav Ambwani.
________________________________
From: Gaurav Ambwani <g...@st-andrews.ac.uk><mailto:g...@st-andrews.ac.uk>
Sent: Wednesday, June 26, 2024 2:12 PM
To: Freesurfer support list 
<freesurfer@nmr.mgh.harvard.edu><mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] MRI watershed error

Dear Freesurfer team,

The issue with that is that on fsl I would need to skull strip the image that I 
want to input for the registration. The reference image doesn't have to be 
skull stripped, but the input image has to be skull stripped.

When I take the skull stripped image as my reference image and input the T1 MNI 
image, the registration isn't successful, i.e. the image seems warped.

Thank you,
Best Wishes,
Gaurav Ambwani.
________________________________
From: 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu><mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 on behalf of Douglas N. Greve 
<dgr...@mgh.harvard.edu><mailto:dgr...@mgh.harvard.edu>
Sent: Tuesday, June 25, 2024 9:51 PM
To: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu> 
<freesurfer@nmr.mgh.harvard.edu><mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] MRI watershed error

you can compute the registration with the skull-stripped image, and then apply 
the registration to the whole head image
On 6/25/2024 12:31 PM, Gaurav Ambwani wrote:

        External Email - Use Caution
Dear Freesurfer team,

I can't put the whole head into FSL for registration, I have to put the skull 
stripped version into FSL. I have to put in the skull stripped head because the 
MNI_freesurfer_T1.nii.gz  is registered to the stroke patient's T1 weighted 
image.

Using the I want the aseg, aparc, etc. files to be derived from the 
MNI_freesurfer_T1.nii.gz image. And for some reason it isn't working. I hope 
that this clarifies what I was trying to do.

Thank you,
Best Wishes,
Gaurav Ambwani.
________________________________
From: 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu><mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 on behalf of Douglas N. Greve 
<dgr...@mgh.harvard.edu><mailto:dgr...@mgh.harvard.edu>
Sent: Monday, June 24, 2024 9:50 AM
To: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu> 
<freesurfer@nmr.mgh.harvard.edu><mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] MRI watershed error

Sorry, I'm not sure what the problem is that you are describing. I see from the 
recon-all file that

MNI_freesurfer_T1.nii.gz is your input. What is that? Is that after skull 
stripping? Why not just give it the full head?
On 6/20/2024 3:52 PM, Gaurav Ambwani wrote:

        External Email - Use Caution
Dear Freesurfer developers,

I took a 1mm T1 MRI of the MNI152 skull stripped it using HD brain extraction 
tool in 3D slicer, and then I registered it in fsl to a skull stripped version 
of a T1 weighted image of a Stroke patient (which I got from Openneuro).

The purpose of doing this is to, get the aseg.mgz file and overlay that onto 
the MRI of the stroke patient.

Previously I have taken the T1 1mm MNI image and received an aseg.mgz file 
which I have overlayed onto my MNI registered T1 weighted stroke MRI and that 
worked perfectly. However, This time I have taken the T1 1mm MNI image 
registered that to my stroke T1 MRI, and the registered image hasn't gone 
through freesurfer.

I have attached the recon-all.log file in this email.

Freesurfer version: freesurfer-linux-ubuntu22_x86_64-7.4.1-20230614-7eb8460
Uname -a: Linux DESKTOP-HV9KQQ6 5.10.16.3-microsoft-standard-WSL2 #1 SMP Fri 
Apr 2 22:23:49 UTC 2021 x86_64 x86_64 x86_64 GNU/Linux
Distributor ID: Ubuntu
Description:    Ubuntu 22.04.4 LTS
Release:        22.04
Codename:       jammy

I am using a WSL in windows 11 (64 bit)

Thank you,
Best Wishes,
Gaurav Ambwani.



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