Hi,

I have encountered the same kind of errors. When I update the loc files
link to GATK, some of the tools display the reference genomes I added and
some not. It seems that the galaxy wrapper for GATK 1.6 is not very
functional. GATK don't really care because they are not supporting it any
more, even documentation has disappeared. And I understand that galaxy
developers have other stuff to do than supporting a tool that
will disappear because it's not open source any more. I don't know what
tool could replace the recalibration process done by GATK and don't know
how to correct bugs neither. Any suggestions ?

Philippe



2012/12/12 Farooq,Umar (res) <ufar...@resident.uchc.edu>

> Hi,
>
> I am trying to incorporate GATK in my pipeline but not been able to make
> it work. I aligned my data with Hg 19 and then ran sam tool filter and then
> picard duplicate removal. I uploaded dbSNP and the reference FASTA file for
> Hg 19 in galaxy to run this pipeline. But for some reason GATK tool for
> base recalibration will not accept this output file. I wonder if there is
> sorting or indexing issue but how to fix this in galaxy.
>
>
> An error occurred running this job: Picked up _JAVA_OPTIONS:
> -Djava.io.tmpdir=/space/g2main [Mon Dec 10 10:30:42 EST 2012]
> net.sf.picard.sam.CreateSequenceDictionary
> REFERENCE=/space/g2main/tmp-gatk-tKp41A/gatk_input.fasta
> OUTPUT=/space/g2main/tmp-gatk-tKp41A/dict3503196447953523717.tmp
>
> Thanks,
> Umar
>
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