Hi,
Sice my reads are just 32bp long, I am trying to use blat instead of blastx to
map them into proteins.
I am trying to run
blat nr.fa seqsaglobus.fa ris2.psl -t=prot -q=dnax -tileSize=8 -stepSize=3 -
fine -repMatch=1000000 -out=blast8
But I got the message:
d and q must both be either protein or dna
I tried with
-t=prot -q=prot
and I get some results, but it should not work because seqsaglobus.fa is made
by nucleotides.
Can you explain me what happens?
Thank you,
Fabio
--
F. Gori, PhD student
Intelligent Systems
ICIS (Institute for Computing and Information Sciences)
Radboud University Nijmegen
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