Hello Tapas,

These methods are extracting data from two sources. One is the backend 
data table, the other the browser display. Coordinates in the data table 
use a "0-based" start position - add +1 to get the "1-based" start 
position, as described in this FAQ:

Database/browser start coordinates differ by 1 base
http://genome.ucsc.edu/FAQ/FAQtracks.html#tracks1

When examining data aligned to the reverse strand, the coordinates in 
the data table are still organized smallest->largest genome coordinate. 
Meaning, the stop (smallest coordinate) will have the +1 added. This can 
be a bit confusing, but if you are interested in the details, this 
additional FAQ can be helpful to review:

Coordinate Transforms
http://genomewiki.ucsc.edu/index.php/Coordinate_Transforms

Please feel free to contact the mailing list support team again if you 
would like more assistance.

Warm regards,

Jen
UCSC Genome Browser Support

On 8/3/10 11:36 PM, [email protected] wrote:
> Respected Sir,
>            I am a research fellow at Indian Statistical Institute, Kolkata,
> India. I am currently involved in Bio-informatics research.
> Currently I am searching for chromosomal location of microRNAs.
>
>            I have found that there is two ways that gives the desired
> information. First way to find required information is ->  (i)
> goto  http://genome.ucsc.edu/cgi-bin/hgTables , (ii) put clade :
> Mammal, genome : Human, assembly : Mar. 2006 (NCBI36/hg18),
> group:  Genes and Gene Prediction Tracks, track :  sno/miRNA ,
> table:  wgRna .
>            Second one is ->  i) goto
> http://genome.ucsc.edu/cgi-bin/hgGateway, (ii) put clade:
> Mammal, genome: Human, assembly: Mar. 2006 (NCBI36/hg18),
> position: genename (user input), gene: blank, image width:
> 800(default).
>
>            For example : miRNA - hsa-mir-216a
> 1st one gives : chr = 2 , chromStart = 56069588       chromEnd = 56069698
> 2nd one gives: chr2:56,069,589-56,069,698.
>
>            But the 2 ways gives everything right except chromStart. If
> first one gives its value equal to x then second one gives its
> value equal to x+1. Please give me the information regarding
> which one is correct
>
>             Thanking you,
>                                                    Yours faithfully,
>                                                    Tapas Bhadra
>                                                    Senior Research Fellow,
>                                                    ISI Kolkata
>
> This mail is scanned by Ironport
>
>
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