Hi Greg,

Your suggestion of adding the search type parameter was perfect.

Thank you,
Jake

On Mon, May 9, 2011 at 12:38 PM, Greg Roe <[email protected]> wrote:

> Hi Jake,
>
> I believe you just need to specify the search type in your URL (e.g.
> type=DNA).  It should then work.
>
> Please see this previous mailing list question for extra details:
> https://lists.soe.ucsc.edu/pipermail/genome/2006-June/011020.html
>
> - Greg
>
>
>
> On 5/6/11 8:28 PM, jake lin wrote:
>
>> Hi,
>>
>> The lab I am supporting would like to link our web content repository to
>> UCSC BLAT or NCBI BLAST web functions. It is an internal web so the usage
>> will be quite light, couple times a day at most, looking up oligo sequence
>> by creating these oligo sequences into html links, setting the query
>> parameter.
>>
>> Our preference is to use BLAT, so much faster and the nicety of getting
>> the
>> results on the genome browser. Also the insillico PCR function.
>>
>> As the BLAT web ui loads, I see that I can construct the organism, and sub
>> db preference within the url/browser links:
>> ie loading C. elegans and db ce4
>> http://genome.ucsc.edu/cgi-bin/hgBlat?org=C.%20elegans&db=ce4
>>
>> But when I try passing in a sequence, looks to be userSeq
>>
>> http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=C.%20elegans&db=ce4&userSeq=AATTAATTAATTAATTAATT
>>
>> Here is the output from webpage:
>>
>> Sorry, didn't find input variable type Probably the web page didn't mean
>> to
>> call this program.
>> Am I doing something silly?
>> Looks like the insillico PCR allows passing in sequence, as does the
>> BLAST.
>>
>> Thanks,
>> Jake
>>
>>


-- 
jl
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