Hello- I am planning to sequence the transcriptome of 700 human lymphocyte samples. In order to calculate the number of samples to run per lane to achieve 30X coverage, I need to determine the size of the human transcriptome. Is it correct to use the number of block bases (71,851,762) listed in the RefSeq table for the Feb 2009 (GRCh37/hg19) assembly?
Thanks so much for your help! Kim ____________________________________ Kimberly D. Spradling, Ph.D. Postdoctoral Scientist, Department of Genetics Texas Biomedical Research Institute 7620 NW Loop 410 San Antonio, TX 78254 Phone: 210-258-9624 _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
