In ideal point, ~2% of human genome is exon. So human transcriptome should be ~60M. Our recently results show ~4% of human genome are transcribed, so human transcriptome indeed should be ~120M. I hope this will help you.
Mingfeng On Tue, Sep 27, 2011 at 7:53 PM, Kim Spradling <[email protected]> wrote: > Hello- > > I am planning to sequence the transcriptome of 700 human lymphocyte > samples. In order to calculate the number of samples to run per lane > to achieve 30X coverage, I need to determine the size of the human > transcriptome. Is it correct to use the number of block bases > (71,851,762) listed in the RefSeq table for the Feb 2009 (GRCh37/hg19) > assembly? > > Thanks so much for your help! > Kim > > ____________________________________ > Kimberly D. Spradling, Ph.D. > Postdoctoral Scientist, Department of Genetics > Texas Biomedical Research Institute > 7620 NW Loop 410 > San Antonio, TX 78254 > Phone: 210-258-9624 > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > -- ********************************************* Mingfeng Li, Ph.D. Postdoctoral Associate Department of Neurobiology Yale University School of Medicine 333 Cedar Street, SHM C-327C New Haven, CT 06510 E-mail: [email protected] Lab: (203) 785-5941 Lab website: www.sestanlab.org ********************************************* _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
